HeLa whole cell lysate (ab29545)
製品の概要
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製品名
HeLa whole cell lysate
HeLa ライゼート 製品一覧 -
特記事項
Protein is isolated from HeLa cell pellet using a proprietary technique. The pellet was frozen in liquid nitrogen immediately and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Allow to warm to room temp and agitate gently before aliquotting. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.06% Potassium chloride, 0.03% Magnesium chloride, 0.4% HEPES, 1.2% Sucrose, 1.8% Glycerol (glycerin, glycerine), 2.5% EDTA, 0.5% Sodium deoxycholate
With Protease inhibitor cocktail/EDTA/Sodium deoxycholate -
Concentration information loading...
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ライゼート 備考
Protein is isolated from HeLa cell pellet using a proprietary technique. The pellet was frozen in liquid nitrogen immediately and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. -
研究分野
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背景
HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.
関連製品
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Recombinant Protein
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Related Products
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab29545の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.
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特記事項 |
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WB
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab29545 は 2 報の論文で使用されています。
- Vonk PJ & Ohm RA H3K4me2 ChIP-Seq reveals the epigenetic landscape during mushroom formation and novel developmental regulators of Schizophyllum commune. Sci Rep 11:8178 (2021). PubMed: 33854169
- Meng H et al. L450W and Q455K Col8a2 knock-in mouse models of Fuchs endothelial corneal dystrophy show distinct phenotypes and evidence for altered autophagy. Invest Ophthalmol Vis Sci 54:1887-97 (2013). PubMed: 23422828