Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

HeLa whole cell lysate (ab150035)

Submit a review Q&A (1)
Western blot - HeLa whole cell lysate (ab150035)
  • Western blot - HeLa whole cell lysate (ab150035)
  • SDS-PAGE - HeLa whole cell lysate (ab150035)
  • Western blot - HeLa whole cell lysate (ab150035)
  • Western blot - HeLa whole cell lysate (ab150035)

製品の概要

  • 製品名

    HeLa whole cell lysate
    HeLa (Human epithelial carcinoma cell line) ライゼート 製品一覧

  • 特記事項

    We recommend aliquoting the extracts into single use fractions and then storing them at -80°C.

     

    This HeLa whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT).

  • アプリケーション

    適用あり: WBmore details

製品の特性

  • Mycoplasma free

    Yes

  • 製品の状態

    Liquid

  • 保存方法

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.50
    Constituent: 10% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
  • Concentration information loading...

  • ライゼート 備考

    This HeLa whole cell was prepared using a standard whole cell lysate protocol. 

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab150035の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.

画像

  • Western blot - HeLa whole cell lysate (ab150035)
    Western blot - HeLa whole cell lysate (ab150035)
    All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Observed band size: 42 kDa why is the actual band size different from the predicted?

  • Western blot - HeLa whole cell lysate (ab150035)
    Western blot - HeLa whole cell lysate (ab150035)
    All lanes : Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (ab63766) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • SDS-PAGE - HeLa whole cell lysate (ab150035)
    SDS-PAGE - HeLa whole cell lysate (ab150035)
    All lanes  stained with Optiblot Blue (ab119211) (20 ml)

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)

    Lysates/proteins at 10 µg per lane.
  • Western blot - HeLa whole cell lysate (ab150035)
    Western blot - HeLa whole cell lysate (ab150035)
    All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

    Observed band size: 51 kDa why is the actual band size different from the predicted?

  • Western blot - HeLa whole cell lysate (ab150035)
    Western blot - HeLa whole cell lysate (ab150035)
    All lanes : Anti-HDAC1 antibody (ab19845) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Observed band size: 60 kDa why is the actual band size different from the predicted?

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab150035 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab150035 は論文での使用が確認できていません。

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Question

Dear recipient,
Does the Dkk1 antibody ab 61275 include the positive control mentioned
in the data sheet (HeLa cell lysate)? Or should I purchase it
separately?
Thank you very much for your reply.
Best regards,

Read More
Answer

Thank you for contacting us.

I can confirm that the positive control is not included into an order of the DKK antibody ab61275.

However, we do offer two products of HeLa cell lysate: ab29545 (tested for WB application) and ab150035 that can be used as positive controls for WB. For further information about both products use the provided links below.

ab29545
https://www.abcam.com/HeLa-whole-cell-lysate-ab29545.html (or use the following: https://www.abcam.com/HeLa-whole-cell-lysate-ab29545.html).


ab150035
https://www.abcam.com/HeLa-whole-cell-lysate-ab150035.html (or use the following: https://www.abcam.com/HeLa-whole-cell-lysate-ab150035.html).


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com