E. coli Positive Control (Escherichia coli ) Whole Cell Lysate

7 Images

• WB

Project

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (AB5395)

Abcam recommends using 3% milk as the blocking agent.

All lanes:

Western blot - HRP Anti-HA tag antibody (<a href='/products/primary-antibodies/hrp-ha-tag-antibody-ab128131'>ab128131</a>) at 1/5000 dilution

Lane 1:

HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) at 10 µg

Lane 2:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

Predicted band size: 64 kDa

Observed band size: 34 kDa,48 kDa,55 kDa

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Exposure time: 150s

• WB

Ap

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (AB5395)

All lanes:

Western blot - HRP Anti-Myc tag antibody [9E10] (<a href='/products/primary-antibodies/hrp-myc-tag-antibody-9e10-ab62928'>ab62928</a>) at 1/10000 dilution

All lanes:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

Predicted band size: 49 kDa

Observed band size: 41 kDa

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• WB

Project

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (AB5395)

This WB was performed using an E. coli lysate that expresses a 41kDa recombinant protein comprised of 12 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.

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• WB

Lab

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (AB5395)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202004 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-6X His tag® antibody [4D11] (<a href='/products/primary-antibodies/hrp-6x-his-tag-antibody-4d11-ab202004'>ab202004</a>) at 1/5000 dilution

All lanes:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

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Exposure time: 3min

• WB

Project

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (AB5395)

This image was produced using the same antibody clone but different formulation, ab32.

Lysate from E. coli recombinantly expressing 11 commonly used tags including myc tag.

All lanes:

Western blot - Anti-Myc tag antibody [9E10] (<a href='/products/primary-antibodies/myc-tag-antibody-9e10-ab32'>ab32</a>) at 1 µg/mL

All lanes:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 48 kDa,49 kDa

Observed band size: 45 kDa

true

Exposure time: 1min

• WB

Unknown

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (AB5395)

false

• WB

Lab

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (AB5395)

All lanes:

Western blot - Anti-Myc tag antibody [9E11] (<a href='/products/primary-antibodies/myc-tag-antibody-9e11-ab56'>ab56</a>) at 1 µg/mL

Lane 1:

HEK293 cell lysate at 20 µg

Lane 2:

Western blot - Recombinant Human c-Myc protein (<a href='/products/proteins-peptides/recombinant-human-c-myc-protein-ab84132'>ab84132</a>) at 0.1 µg

Lane 3:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG H&L Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 49 kDa

false