E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
製品の概要
-
製品名
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate -
特記事項
This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:
GST
T7
HSV
C-Myc
VSV-g
Glu-Glu
V5
E-tag
FLAG (DDDDK)
S-tag
HA
6x His
Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.
-
アプリケーション
適用あり: WBmore details
製品の特性
-
Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
1X Loading Buffer (1X SDS-PAGE Sample Buffer: 62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue) -
Concentration information loading... -
ライゼート 備考
Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.
-
研究分野
-
背景
Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5395の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
Use 0.5 - 2.0 µl for 10 - 12% gels. |
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Use 0.5 - 2.0 µl for 10 - 12% gels. |
画像
-
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)All lanes : Anti-6X His tag® antibody (ab9108) at 1/1000 dilution
Lane 1 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 2 µl
Lane 2 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 1 µl
Lane 3 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.5 µl
Lane 4 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.25 µl -
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)This WB was performed using an E. coli lysate that expresses a 41kDa recombinant protein comprised of 12 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab5395 は 3 報の論文で使用されています。
- Shokri L et al. A Comprehensive Drosophila melanogaster Transcription Factor Interactome. Cell Rep 27:955-970.e7 (2019). PubMed: 30995488
- Májek P et al. Improved Coomassie Blue Dye-Based Fast Staining Protocol for Proteins Separated by SDS-PAGE. PLoS One 8:e81696 (2013). PubMed: 24278455
- Miriam_Sans, Itzel_Vargas-Prez, Luis_Quintales, Fr Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res : 1-11 (2011). WB ; Schizosaccharomyces pombe . PubMed: 21515633