CD147 overexpression 293T lysate (whole cell) (ab94097)
製品の概要
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製品名
CD147 overexpression 293T lysate (whole cell)
CD147 ライゼート 製品一覧 -
特記事項
ab94097 is a 293T cell transfected lysate in which Human CD147 has been transiently over-expressed using a pCMV-CD147 plasmid. The lysate is provided in 1X Sample Buffer.
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アプリケーション
適用あり: WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
研究分野
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背景
Function: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Tissue specificity: Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. PTM: N-glycosylated.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94097の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
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WB
Use at an assay dependent dilution. |
画像
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SDS-PAGE - CD147 overexpression 293T lysate (whole cell) (ab94097)ab94097 at 15µg/lane on an SDS-PAGE gel.
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Western blot - CD147 overexpression 293T lysate (whole cell) (ab94097)All lanes : Anti-CD147 antibody (ab88530) at 1/500 dilution
Lane 1 :CD147 overexpression 293T lysate (whole cell) (ab94097)
Lane 2 : 293T Non transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab94097 は論文での使用が確認できていません。