AB276092

Human TP53 (p53) knockout A549 cell line

Advanced Validation
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(3 Publications)

TP53 KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 49 bp deletion in exon 4. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.

別名を表示する

Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, P53_HUMAN, Phosphoprotein p53, TRP53, Tp53, Transformation related protein 53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53, p53 tumor suppressor, tumor antigen p55

4 Images

Lab

Western blot - Human TP53 (p53) knockout A549 cell line (AB276092)

False colour image of Western blot : Anti-p53 antibody [DO-1] - ChIP Grade staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab1101 was shown to bind specifically to p53. A band was observed at 40/50 kDa in treated wild-type A549 cell lysates with no signal observed at this size in tp53 knockout cell line ab276092. To generate this image, wild-type and tp53 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

Lanes 1 - 12:

Western blot - Anti-p53 antibody [DO-1] (<a href='/products/primary-antibodies/p53-antibody-do-1-chip-grade-ab1101'>ab1101</a>) at 1/1000 dilution

Lanes 1 - 12:

Western blot - Anti-p53 antibody [DO-1] - BSA and Azide free (<a href='/products/primary-antibodies/p53-antibody-do-1-bsa-and-azide-free-ab237976'>ab237976</a>)

Lane 1:

MCF7 Treated Camptothecin (20 ug/ml, 0 h) cell lysate at 15 µg

Lane 2:

MCF7 Treated Camptothecin (20 ug/ml, 6 h) cell lysate at 15 µg

Lane 3:

MCF7 Treated Camptothecin (20 ug/ml, 24 h) cell lysate at 15 µg

Lane 4:

Wild-type A549 Treated Camptothecin (20 ug/ml, 0 h) cell lysate at 15 µg

Lane 5:

Wild-type A549 Treated Camptothecin (20 ug/ml, 6 h) cell lysate at 15 µg

Lane 6:

Wild-type A549 Treated Camptothecin (20 ug/ml, 24 h) cell lysate at 15 µg

Lane 7:

TP53 knockout A549 Treated Camptothecin (20 ug/ml, 0 h) cell lysate at 15 µg

Lane 8:

TP53 knockout A549 Treated Camptothecin (20 ug/ml, 6 h) cell lysate at 15 µg

Lane 9:

TP53 knockout A549 Treated Camptothecin (20 ug/ml, 24 h) cell lysate at 15 µg

Lane 10:

Saos-2 cell lysate at 15 µg

Lane 11:

SK-BR-3 cell lysate at 15 µg

Lane 12:

A431 cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/20000 dilution

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Lab

Western blot - Human TP53 (p53) knockout A549 cell line (AB276092)

False colour image of Western blot : Anti-p53 antibody [DO-1] - ChIP Grade staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab1101 was shown to bind specifically to p53. A band was observed at 49 kDa in wild-type A549 cell lysates with no signal observed at this size in tp53 knockout cell line ab276092 (knockout cell lysate ab282999). To generate this image, wild-type and tp53 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-p53 antibody [DO-1] (<a href='/products/primary-antibodies/p53-antibody-do-1-chip-grade-ab1101'>ab1101</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human TP53 (p53) knockout A549 cell line (ab276092) at 20 µg

Lane 3:

Wild-type Jurkat cell lysate at 20 µg

Lane 4:

Western blot - Human TP53 (p53) knockout Jurkat cell line (<a href='/products/cell-lines/human-tp53-p53-knockout-jurkat-cell-line-ab276112'>ab276112</a>) at 20 µg

Lane 5:

A431 cell lysate at 20 µg

Lane 6:

Saos-2 cell lysate at 20 µg

Predicted band size: 43 kDa

Observed band size: 49 kDa

false

Lab

Western blot - Human TP53 (p53) knockout A549 cell line (AB276092)

False colour image of Western blot : Anti-p53 antibody [E26] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32389 was shown to bind specifically to p53. A band was observed at 49 kDa in wild-type A549 cell lysates with no signal observed at this size in tp53 knockout cell line ab276092 (knockout cell lysate ab282999). To generate this image, wild-type and tp53 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-p53 antibody [E26] (<a href='/products/primary-antibodies/p53-antibody-e26-ab32389'>ab32389</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

TP53 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human TP53 (p53) knockout Jurkat cell line (<a href='/products/cell-lines/human-tp53-p53-knockout-jurkat-cell-line-ab276112'>ab276112</a>)

Lane 3:

Wild-type Jurkat cell lysate at 20 µg

Lane 4:

TP53 knockout Jurkat cell lysate at 20 µg

Lane 5:

A431 cell lysate at 20 µg

Lane 6:

Saos-2 cell lysate at 20 µg

Predicted band size: 43 kDa

Observed band size: 49 kDa

false

Sanger Sequencing - Human TP53 (p53) knockout A549 cell line (AB276092)

Sanger seq

Lab

Sanger Sequencing - Human TP53 (p53) knockout A549 cell line (AB276092)

49 bp deletion in exon 4

Key facts

細胞タイプ

A549

生物種

Human

組織

Lung

製品の状態

Liquid

form

ノックアウト検証方法

Sanger Sequencing

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, Homozygous: 49 bp deletion in exon 4

疾病

Carcinoma

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Reactivity data

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製品の詳細

We will provide viable cells that proliferate on revival.

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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製品内容

{ "values": { "2x1000000Cellsvial": { "sellingSize": "2 x 1000000 Cells/vial", "publicAssetCode":"ab276092-2x1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab275463 Human wild-type A549 cell line", "number":"AB276092-CMP02" }, { "size":"1 x 1000000 Cells/vial", "name":"ab276092 Human TP53 (p53) knockout A549 cell line", "number":"AB276092-CMP01" } ] }, "1000000Cellsvial": { "sellingSize": "1000000 Cells/vial", "publicAssetCode":"ab276092-1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab276092 Human TP53 (p53) knockout A549 cell line", "number":"AB276092-CMP01", "productcode":"" } ] } } }

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出荷温度及び保存条件

遺伝子名

TP53

遺伝子編集のタイプ

Knockout

遺伝子編集の方法

CRISPR technology

ノックアウト検証方法

Sanger Sequencing

接合型

Homozygous

出荷温度

Dry Ice

短期保存温度

-196°C

長期保存温度

-196°C

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取り扱い方法

初回取り扱いガイドライン

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO₂. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x10⁴ cells/cm². Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

継代培養ガイドライン

All seeding densities should be based on cell counts gained by established methods.
A guide seeding density of 2x10⁴ cells/cm² is recommended.
Cells should be passaged when they have achieved 80-90% confluence.
Do not allow the cell density to exceed 7x10⁴ cells/cm².

培養培地

F-12K + 10% FBS

凍結保存培地

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information TP53

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Genome research 35:1456-1471 PubMed40360186

2025

Charting the regulatory landscape of TP53 on transposable elements in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Qu,Yonghao Liang,Colin McCornack,Xiaoyun Xing,Heather Schmidt,Chad Tomlinson,Catrina Fronick,Edward A Belter,Juan F Macias-Velasco,Ting Wang

Evidence-based complementary and alternative medicine : eCAM 2023:8378581 PubMed36814470

2023

Curcumin Analog, HO-3867, Induces Both Apoptosis and Ferroptosis via Multiple Mechanisms in NSCLC Cells with Wild-Type p53.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wu,Guodong Xu,Ni Li,Linwen Zhu,Guofeng Shao

Nature cell biology 24:1099-1113 PubMed35798843

2022

A p53-phosphoinositide signalosome regulates nuclear AKT activation.

Applications

Unspecified application

Species

Unspecified reactive species

Mo Chen,Suyong Choi,Tianmu Wen,Changliang Chen,Narendra Thapa,Jeong Hyo Lee,Vincent L Cryns,Richard A Anderson

View all publications

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Human TP53 (p53) knockout A549 cell line