Human PARP10 (ARTD10) knockout A549 cell line
Human PARP10 (ARTD10) knockout A549 cell line
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PARP10 KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 5 and 1 bp insertion in exon 5 and 4 bp deletion in exon 5. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
別名を表示する
ADP ribosyltransferase diphtheria toxin like 10, PARP 10, Poly (ADP ribose) polymerase family member 10, Poly [ADP ribose] polymerase 10
- Sanger seq
Unknown
Sanger Sequencing - Human PARP10 (ARTD10) knockout A549 cell line (AB267161)
Allele-2 : 1 bp insertion in exon 5.
- Sanger seq
Unknown
Sanger Sequencing - Human PARP10 (ARTD10) knockout A549 cell line (AB267161)
Allele-3 : 4 bp deletion in exon 5.
- Sanger seq
Unknown
Sanger Sequencing - Human PARP10 (ARTD10) knockout A549 cell line (AB267161)
Allele-1 : 1 bp deletion in exon5
製品の詳細
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
製品内容
出荷温度及び保存条件
遺伝子名
遺伝子編集のタイプ
遺伝子編集の方法
ノックアウト検証方法
出荷温度
短期保存温度
長期保存温度
取り扱い方法
初回取り扱いガイドライン
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
継代培養ガイドライン
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
培養培地
F-12K + 10% FBS
凍結保存培地
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ARTD10 influences several cellular processes including transcriptional regulation and cell cycle progression. It functions independently without being part of a stable protein complex but interacts with other nuclear and cytoplasmic proteins to exert its role. This protein is involved in modulating cellular responses to DNA damage and stress impacting cellular homeostasis and metabolism. Its interaction patterns highlight its significance in cellular regulatory mechanisms.
Pathways
ARTD10 is an active participant in DNA repair and stress response pathways. It is closely linked with the NF-kB signaling pathway where it modulates inflammation and cell survival outcomes. ARTD10 also connects with proteins like PARP1 sharing a broader role in the maintenance of genomic integrity and response to DNA insults. These pathways underpin the critical mechanisms by which cells manage and adapt to damages and maintain cellular viability.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
ターゲットの情報
組合せ製品
Abcam product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com