Human MRPL2 knockout HEK-293T cell line
Human MRPL2 knockout HEK-293T cell line
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MRPL2 KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 100 bp deletion in exon 1 and 43 bp insertion in exon 1 and 7 bp deletion in exon 1. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
別名を表示する
39S ribosomal protein L2, 39S ribosomal protein L2, mitochondrial precursor, CGI22, L2mt, Mitochondrial ribosomal protein L2, RM02_HUMAN, RPML14, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human MRPL2 knockout HEK-293T cell line (AB266698)
Allele-1 : 100 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human MRPL2 knockout HEK-293T cell line (AB266698)
Allele-2 : 7 bp deletion in exon 1.
- Sanger seq
Unknown
Sanger Sequencing - Human MRPL2 knockout HEK-293T cell line (AB266698)
Allele-3 : 43 bp insertion in exon 1.
製品の詳細
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
製品内容
出荷温度及び保存条件
遺伝子名
遺伝子編集のタイプ
遺伝子編集の方法
ノックアウト検証方法
出荷温度
短期保存温度
長期保存温度
取り扱い方法
初回取り扱いガイドライン
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
継代培養ガイドライン
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
培養培地
DMEM (High Glucose) + 10% FBS
凍結保存培地
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MRPL2 contributes to efficient protein manufacturing within the mitochondria by being a part of the mitochondrial ribosomal complex. This complex is responsible for translating 13 essential polypeptides that integrate into the inner mitochondrial membrane and are important for oxidative phosphorylation. MRPL2's presence ensures the translation process occurs smoothly directly influencing cellular energy production. The integrity of mitochondrial protein synthesis significantly affects overall cellular metabolism and energy balance.
Pathways
MRPL2 influences mitochondrial translation and oxidative phosphorylation. It works within pathways that maintain cellular energy production directly impacting cell survival and function. During these processes MRPL2 interacts with essential components like mitochondrial ATP synthase and cytochrome c oxidase facilitating energy conversion from food into usable ATP. These close interactions demonstrate MRPL2's importance in sustaining energy homeostasis and cellular respiration.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
ターゲットの情報
組合せ製品
Abcam product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com