AB266123

Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line

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GOLIM4 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 1. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.

別名を表示する

130 kDa golgi localized phosphoprotein, CIS, GIMPc, GOLI4_HUMAN, GOLPH 4, GPP 130, Golgi integral membrane protein, Golgi integral membrane protein 4, Golgi phosphoprotein 4, Golgi phosphoprotein of 130 kDa, Golgi-localized phosphoprotein of 130 kDa, P 138, cis Golgi localized calcium binding protein, golim4, type II Golgi membrane protein

4 Images

Lab

Western blot - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line (AB266123)

Lanes 1-4 : Merged signal (red and green). Green - ab197595 observed at 130 kDa. Red - loading control ab8245 observed at 36 kDa.

ab197595 Anti-GOLPH4/GPP130 antibody [EPR13439] was shown to specifically react with GOLPH4/GPP130 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266123 (knockout cell lysate ab257455) was used. Wild-type and GOLPH4/GPP130 knockout samples were subjected to SDS-PAGE. ab197595 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4° at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (<a href='/products/primary-antibodies/golph4-gpp130-antibody-epr13439-ab197595'>ab197595</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

GOLIM4 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line (ab266123)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

MOLT-4 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 82 kDa

Observed band size: 130 kDa

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Sanger Sequencing - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line (AB266123)

Sanger seq

Lab

Sanger Sequencing - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line (AB266123)

Sequencing chromatogram displaying sequence edit in exon 1

Sanger seq

Unknown

Sanger Sequencing - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line (AB266123)

Homozygous : 2 bp insertion in exon 1

Cell Culture - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line (AB266123)

Cell Culture

Unknown

Cell Culture - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line (AB266123)

Representative images of GOLIM4 knockout HEK293T cells, low and high confluency examples (top left and right respectively) and wild-type HEK293T cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS XL Core microscope.

Key facts

細胞タイプ

HEK-293T

生物種

Human

組織

Kidney

製品の状態

Liquid

form

ノックアウト検証方法

Sanger Sequencing,Western blot

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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製品内容

{ "values": { "2x1000000Cellsvial": { "sellingSize": "2 x 1000000 Cells/vial", "publicAssetCode":"ab266123-2x1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab266123 Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line", "number":"AB266123-CMP01" }, { "size":"1 x 1000000 Cells/vial", "name":"ab255449 Human wild-type HEK-293T cell line", "number":"AB266123-CMP02" } ] }, "1000000Cellsvial": { "sellingSize": "1000000 Cells/vial", "publicAssetCode":"ab266123-1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab266123 Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line", "number":"AB266123-CMP01", "productcode":"" } ] } } }

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出荷温度及び保存条件

遺伝子名

GOLIM4

遺伝子編集のタイプ

Knockout

遺伝子編集の方法

CRISPR technology

ノックアウト検証方法

Sanger Sequencing, Western blot

接合型

Homozygous

出荷温度

Dry Ice

短期保存温度

-196°C

長期保存温度

-196°C

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取り扱い方法

初回取り扱いガイドライン

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO₂. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x10⁴ cells/cm². Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

継代培養ガイドライン

All seeding densities should be based on cell counts gained by established methods.
A guide seeding density of 2x10⁴ cells/cm² is recommended.
Cells should be passaged when they have achieved 80-90% confluence.

培養培地

DMEM (High Glucose) + 10% FBS

凍結保存培地

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GOLPH4 also known as GPP130 and Golgi integral membrane protein 4 is a protein with a mass of approximately 130 kDa. It plays an important role in the organization and maintenance of the Golgi apparatus structure. GOLPH4 is predominantly found in the cis-Golgi network and it is involved in the cellular sorting and trafficking of proteins and lipids. The protein functions as a tethering factor and it may participate in the transport of cargo from the Golgi to other cellular locations.

Biological function summary

The role of GOLPH4 centers around its involvement in the protein trafficking process within cells. It acts as part of the complex tasked with maintaining Golgi organization which is important for facilitating accurate cargo sorting and delivery. GOLPH4's location and structure enable it to interact with motor proteins and other Golgi matrix proteins aiding in vesicle tethering and fusion processes.

Pathways

GOLPH4 is involved in essential cellular pathways such as the secretion and endocytic trafficking pathways. It interacts with other Golgi-resident proteins like GM130 and giantin which are essential for the movement of materials through the Golgi and to their final destinations. These pathways ensure that proteins and lipids reach their intended areas within the cell or are secreted to the extracellular space which is important for maintaining cellular function and homeostasis.

Disturbances in GOLPH4 function have been associated with conditions such as cancer and neurodegenerative diseases. Aberrant expression or mutations in this protein can lead to misregulation of Golgi functions affecting overall cellular trafficking systems. GOLPH4's interaction with proteins involved in these conditions such as p53 in cancer or tau in neurodegenerative diseases highlights its potential as a target for therapeutic interventions.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information GOLIM4

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Abcam product promise

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Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line