Human CES1 knockout A549 cell line
Human CES1 knockout A549 cell line
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CES1 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
別名を表示する
ACAT, Acyl coenzyme A cholesterol acyltransferase, Acyl-coenzyme A:cholesterol acyltransferase, Brain carboxylesterase hBr1, CE 1, CEH, CES2, CESDD1, Carboxyesterase ES-3, Carboxylesterase, Carboxylesterase 1, Carboxylesterase 1 (monocyte/macrophage serine esterase 1), Carboxylesterase 1 deficiency, included, Carboxylesterase 2, formerly, Ces-1, Cholesterol ester hydrolase, neutral, macrophage-derived, Cholesteryl ester hydrolase, Cocaine carboxylesterase, EC 3.1.1.1, ES-HTEL, ES-x, EST1_HUMAN, Egasyn, Es22, Esterase, Esterase 22, HMSE, HMSE1, Liver carboxylesterase 1, Liver carboxylesterase 3, MGC117365, MGC156521, Methylumbelliferyl acetate deacetylase 1, Monocyte carboxylesterase deficiency, included, Monocyte esterase deficiency, included, Monocyte/macrophage serine esterase, PCE-1, Proline-beta-naphthylamidase, REH, Retinyl ester hydrolase, Serine esterase 1, Ses-1, TGH, Triacylglycerol hydrolase, hCE 1, pI 5.5 esterase
- NGS
Lab
Next Generation Sequencing - Human CES1 knockout A549 cell line (AB300889)
56 bp deletion after Thr 151 (allele 1); 35 bp deletion after Met 145 (allele 2); 2 bp deletion and 10 bp deletion after Thr 151 (allele 3); 21 bp deletion after Ser 150 and 11 bp deletion after Gln 162 (allele 4) of WT Protein
Reactivity data
製品の詳細
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
製品内容
出荷温度及び保存条件
遺伝子名
遺伝子編集のタイプ
遺伝子編集の方法
ノックアウト検証方法
出荷温度
短期保存温度
長期保存温度
取り扱い方法
初回取り扱いガイドライン
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
継代培養ガイドライン
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
培養培地
F-12K + 10% FBS
凍結保存培地
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
ターゲットの情報
組合せ製品
Abcam product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com