Proteinase K Antigen Retrieval Solution (ab64220)
製品の概要
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製品名
Proteinase K Antigen Retrieval Solution -
アプリケーション
適用あり: IHC-P, In situ hybridizationmore details -
特記事項
ab64220 is a single-component, ready-to-use proteinase K solution for use in protease-induced antigen retrieval during IHC (PIER) and pre-treatment for in situ hybridization.
Staining Protocol:
- IHC procedure: Incubate tissue section for 5 to 7 minutes at room temperature; Rinse and proceed with immunostaining.
- General ISH procedure: Incubate tissue section for 15 minutes at 37oC; Rinse and proceed with hybridization.
Other products for IHC
Other enzymes for antigen retrieval include: Pepsin Solution ab64201 and Trypsin Antigen Retrieval Kit ab259256.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
製品の特性
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製品の状態
Liquid -
保存方法
Store at -20°C. Stable for 12 months at -20°C -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: Tris buffer, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
研究分野
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関連性
Proteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name. Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes. -
別名
- Endopeptidase K
- Tritirachium alkaline proteinase
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64220の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
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| In situ hybridization |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
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IHC-P
Use at an assay dependent dilution. |
|
In situ hybridization
Use at an assay dependent dilution. |
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteinase K (ab64220)ab64220 Proteinase K used with aa anti-Pan CK antibody on Human skin
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab64220 は 27 報の論文で使用されています。
- Lee HI et al. Exosome-mediated delivery of super-repressor IκBα alleviates inflammation and joint damages in rheumatoid arthritis. Arthritis Res Ther 26:2 (2024). PubMed: 38167497
- Kanemaru K et al. Spatially resolved multiomics of human cardiac niches. Nature 619:801-810 (2023). PubMed: 37438528
- Yurtal Z et al. The Matrix Metalloproteinase Inhibitor Batimastat Reduces Epidural Fibrosis After Laminectomy in Rats. Turk Neurosurg 33:162-170 (2023). PubMed: 36482856
- Adam K & Mor A Immunohistochemistry of Immune Cells and Cells Bound to in vivo Administered Antibodies in Liver, Lung, Pancreas, and Colon of B6/lpr Mice. Bio Protoc 12:N/A (2022). PubMed: 35978576
- Yoshii D et al. An autopsy case of adult-onset neuronal intranuclear inclusion disease with perivascular preservation in cerebral white matter. Neuropathology 42:66-73 (2022). PubMed: 34954850