Normal Goat Serum
Normal Goat Serum
5
(3 Reviews)
|
(482 Publications)
- Alternative to bovine serum albumin (BSA) and non-fat dry milk
- Compatible with our Lab essentials imaging workflow offering
- Available in different sizes to fit your experimental needs
- Proven performance: cited in over 480 publications
- IHC-Fr
PubMed
Immunohistochemistry (Frozen sections) - Normal Goat Serum (AB7481)
Slides were pretreated with Hydrogen Peroxide Block followed by 15% normal goat serum (Abcam, Cambridge, UK) for 1 hour. The primary antibody, anti-PTX3, N-terminal antibody produced in rabbit was diluted 1 : 300 in PBS with 2.5% goat serum (ab7481), applied to slides and incubated for 3 hours at 4°C. The primary antibody was omitted in the negative controls. The PTX3 binding was revealed using a universal secondary antibody polymer formulation conjugated to horseradish peroxidase (HRP). The HRP activity was subsequently visualized with diaminobenzidine (DAB) substrate/chromogen and counterstaining with hematoxylin was performed.
Witasp, Anna et al., PloS one vol. 8,5 e63493., Fig 3, doi:10.1371/journal.pone.0063493
- ICC/IF
PubMed
Immunocytochemistry/ Immunofluorescence - Normal Goat Serum (AB7481)
Cells grown in 8-well chamber slides to 70% confluence were fixed with 4% paraformaldehyde (15 min at room temperature (RT)), and permeabilized with 100% ice cold methanol (10 min at 20°C). The slides were incubated with 10% normal goat serum (ab7481) in PBS for 1 hr, and incubated with anti-maspin (1 : 100) antibody alone or in a combination with either anti-lamin B (1 : 50), anti-HDAC1 (1 : 50) or anti-GRP78 (1 : 50) at 4°C overnight. Cells were washed and incubated for 2 hrs at room temperature (RT) with Alexa Fluor 488 (1 : 500) alone or in combination with Alexa Fluor 594 (1 : 500). The nuclei were counterstained with DAPI.
DU145 cells infected with adenovirus expressing either maspinWT or maspinD346E Confocal immunofluorescence imaging of maspin (green) and nuclear envelope marker lamin B (red) in DU145 cells after infection.
Dzinic, Sijana H et al., PloS one vol. 8,11 e74502., Fig 2, doi:10.1371/journal.pone.0074502
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文献 (482)
Recent publications for all applications. Explore the full list and refine your search
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Scientific reports 15:18734 PubMed40436929
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Nature communications 16:4743 PubMed40399268
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Journal, genetic engineering & biotechnology 23:100496 PubMed40390503
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Cancer medicine 14:e70959 PubMed40387385
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BMC veterinary research 21:349 PubMed40380182
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Nature communications 16:4353 PubMed40348760
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Chemical biology & drug design 105:e70116 PubMed40346910
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AB138478
Normal Goat Serum (Sterile)
blood-derived-fluids
normal-goat-serum-sterile-ab138478
0
(0 reviews)
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