Normal Donkey Serum (ab7475)
製品の概要
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製品名
Normal Donkey Serum
Donkey Serum ihc 試薬 製品一覧 -
由来種
Donkey -
アプリケーション
適用あり: IHC (PFA fixed), Blocking, IHC-P, IHC-Fr, ICC/IFmore details -
種交差性
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特記事項
Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.
The donkey serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.
Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised.
Serum can be used directly for blocking, or as a constituent of a blocking buffer.
Strain: Mixed breed and sex.
Raised in: Donkey
Purity: Whole antiserum
製品の特性
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製品の状態
Liquid -
保存方法
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
研究分野
関連製品
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Related Products
- Normal Donkey serum (sterile) (ab138579)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
- Donkey Anti-Goat IgG H&L (HRP) (ab97110)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7475の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC (PFA fixed) |
Use at an assay dependent dilution.
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| Blocking |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
|
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| IHC-Fr |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution.
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| 特記事項 |
|---|
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IHC (PFA fixed)
Use at an assay dependent dilution. |
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Blocking
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution. |
画像
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Functional Studies - Normal Donkey Serum (ab7475)Paul, Rajib et al., PloS one vol. 12,2 e0171285., Fig 5, doi:10.1371/journal.pone.0171285
The sections were rinsed three times with 0.1 M Tris-buffered saline (TBS, 0.1 M; pH 7.4), incubated in 3% H2O2 in TBS, permeabilized with 0.3% Triton X-100, and blocked with 10% donkey serum containing 0.3% Triton X-100. The sections were incubated overnight with primary antibody for TH (1:700) in TBS, containing 2% donkey serum (ab7475) at 4°C and then incubated with HRP-conjugated secondary antibody (1:1000) in TBS for 1 h at room temperature.
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Functional Studies - Normal Donkey Serum (ab7475)Krzy?anowska, Weronika et al., PloS one?vol. 12,10 e0186243., Fig 6, doi:10.1371/journal.pone.0186243
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (193)
ab7475 は 193 報の論文で使用されています。
- Denaro S et al. Sigma-1 Receptor Inhibition Reduces Mechanical Allodynia and Modulate Neuroinflammation in Chronic Neuropathic Pain. Mol Neurobiol 61:2672-2685 (2024). PubMed: 37922065
- Shumliakivska M et al. DNMT3A clonal hematopoiesis-driver mutations induce cardiac fibrosis by paracrine activation of fibroblasts. Nat Commun 15:606 (2024). PubMed: 38242884
- Ko EC et al. Accelerating the in vitro emulation of Alzheimer's disease-associated phenotypes using a novel 3D blood-brain barrier neurosphere co-culture model. Front Bioeng Biotechnol 11:1251195 (2023). PubMed: 37901842
- Rauchenwald T et al. Preadipocytes in human granulation tissue: role in wound healing and response to macrophage polarization. Inflamm Regen 43:53 (2023). PubMed: 37904253
- Zheng M et al. Expression of genes and enzymes involved in ovarian steroidogenesis in relation to human follicular development. Front Endocrinol (Lausanne) 14:1268248 (2023). PubMed: 37964966
