AB7475

Normal Donkey Serum

Name: Normal Donkey Serum (ab7475) | Abcam
Brand: Abcam Limited
SKU: AB7475
Price: 12000 JPY
Availability: InStock

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(268 Publications)

Normal Donkey Serum (ab7475) is used for blocking in antibody-based applications e.g. IHC, ICC/IF or as an antibody diluent. It is effective in reducing nonspecific binding thereby maximizing signal-to-noise ratio.

- Proven performance: cited in over 265 publications
- Available in different sizes to fit your experimental needs
- Compatible with our Lab essentials imaging workflow offering.

2 Images

Immunohistochemistry (PFA perfusion fixed frozen sections) - Normal Donkey Serum (AB7475)

IHC-FoFr

PubMed

Immunohistochemistry (PFA perfusion fixed frozen sections) - Normal Donkey Serum (AB7475)

Anti-xCT rabbit (ab37185 Abcam) dilution : 1 : 200 Donkey anti-rabbit TR (ab6800 Abcam) dilution : 1 : 1000 Donkey normal serum (ab7475 Abcam) dilution : 10%

Krzyzanowska, Weronika et al., PloS one: vol. 12,10 e0186243., Fig 6, doi:10.1371/journal.pone.0186243

Immunohistochemistry (Frozen sections) - Normal Donkey Serum (AB7475)

IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Normal Donkey Serum (AB7475)

The sections were rinsed three times with 0.1 M Tris-buffered saline (TBS, 0.1 M; pH 7.4), incubated in 3% H₂O₂ in TBS, permeabilized with 0.3% Triton X-100, and blocked with 10% donkey serum containing 0.3% Triton X-100. The sections were incubated overnight with primary antibody for TH (1 : 700) in TBS, containing 2% donkey serum (ab7475) at 4°C and then incubated with HRP-conjugated secondary antibody (1 : 1000) in TBS for 1 h at room temperature.

Paul, Rajib et al., PloS one vol. 12,2 e0171285., Fig 5, doi:10.1371/journal.pone.0171285

Key facts

アプリケーション

IHC-P, BL, IHC (PFA fixed), IHC-Fr, ICC/IF

applications

製品の状態

Liquid

form

バッファー組成

Constituents: Whole serum

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at 10% dilution." }, "IHC (PFA fixed)": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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製品の詳細

Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.

The donkey serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.

Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised.

Serum can be used directly for blocking, or as a constituent of a blocking buffer.

Strain: Mixed breed and sex.

Raised in: Donkey

Purity: Whole antiserum

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

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文献 (268)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40571481

2025

Targeting Annexin A2 to reactivate tumor-associated antigens presentation and relieve immune tolerance in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiao Tang,Jianxin Yang,Qicong Shen,Zelong Gao,Mengpu Wu,Chenghua Wu,Jicong Du,Min Li,Changquan Ling,Feng Lu,Yifeng Chai,Xin Dong,Jianxin Qian,Chenqi Li,Feng Xie,Zhenhong Guo,Hui Shen,Dongyao Wang

Journal of neurochemistry 169:e70137 PubMed40542554

2025

The Blue Light-Responsive Lateral Pathway of the Retinohypothalamic Tract Promotes Endocannabinoid-Driven Modulation of Orexin Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola Forte,Roberta Imperatore,Brenda Marfella,Alessandro Nicois,Roberta Verde,Letizia Palomba,Vincenzo Di Marzo,Luigia Cristino

Frontiers in molecular neuroscience 18:1563086 PubMed40496801

2025

Exposure to a nanoplastic-enriched diet for fourteen days increases microglial immunoreactivity in the zebrafish telencephalon.

Applications

Unspecified application

Species

Unspecified reactive species

Robert A Mans,Hannah Kelehear,Sarah Rotschafer,Clare Ganas,Brendan Uche-Moon,Gabrielle Call,Callie C Mauersberg,Justin Toller,Andrew Diamanduros

Nature 644:153-163 PubMed40369074

2025

Spatial transcriptomics reveals human cortical layer and area specification.

Applications

Unspecified application

Species

Unspecified reactive species

Xuyu Qian,Kyle Coleman,Shunzhou Jiang,Andrea J Kriz,Jack H Marciano,Chunyu Luo,Chunhui Cai,Monica Devi Manam,Emre Caglayan,Abbe Lai,David Exposito-Alonso,Aoi Otani,Urmi Ghosh,Diane D Shao,Rebecca E Andersen,Jennifer E Neil,Robert Johnson,Alexandra LeFevre,Jonathan L Hecht,Nicola Micali,Nenad Sestan,Pasko Rakic,Michael B Miller,Liang Sun,Carsen Stringer,Mingyao Li,Christopher A Walsh

Molecular cancer 24:141 PubMed40361105

2025

Profiling triple-negative breast cancer-specific super-enhancers identifies high-risk mesenchymal development subtype and BETi-Targetable vulnerabilities.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Shan Chen,Rui-Zhao Cai,Yan Wang,Ge-Hao Liang,Kai-Ming Zhang,Xiao-Yu Yang,Dong Yang,De-Chang Zhao,Xiao-Feng Zhu,Rong Deng,Jun Tang

Cell 188:3329-3348.e31 PubMed40250419

2025

Fate and state transitions during human blood vessel organoid development.

Applications

Unspecified application

Species

Unspecified reactive species

Marina T Nikolova,Zhisong He,Makiko Seimiya,Gustav Jonsson,Wuji Cao,Ryo Okuda,Reiner A Wimmer,Ryoko Okamoto,Josef M Penninger,J Gray Camp,Barbara Treutlein

Aging cell 24:e70023 PubMed40248988

2025

Deciphering the Contribution of Circular RNAs to Age-Related Decline in Sertoli Cell Survivor.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Manfrevola,Nicola Mosca,Vincenza Grazia Mele,Teresa Chioccarelli,Guillaume Martinez,Charles Coutton,Monica Mattia,Mariaceleste Pezzullo,Silvia Fasano,Gilda Cobellis,Nicoletta Potenza,Rosanna Chianese

Nature cell biology 27:605-618 PubMed40211073

2025

Multipotent neural stem cells originating from neuroepithelium exist outside the mouse central nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Han,Wan Xu,Hyun-Woo Jeong,Hongryeol Park,Kathrin Weyer,Yaroslav Tsytsyura,Martin Stehling,Guangming Wu,Guocheng Lan,Kee-Pyo Kim,Henrik Renner,Dong Wook Han,Yicong Chen,Daniela Gerovska,Marcos J Araúzo-Bravo,Jürgen Klingauf,Jens Christian Schwamborn,Ralf H Adams,Pentao Liu,Hans R Schöler

PloS one 20:e0320605 PubMed40163451

2025

Cytomegalovirus host receptor expression in the human fetal inner ear.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia C M Grijpink,Wouter H van der Valk,Edward S A van Beelen,John C M J de Groot,Heiko Locher,Ann C T M Vossen

BMJ open ophthalmology 10: PubMed40154564

2025

Lymphatic corneal neovascularisation affects graft survival in high-risk corneal transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Nadja Franz,Christoph Palme,Alexander Franchi,Victoria Stöckl,Christof Seifarth,Gertrud Haas,Matus Rehak,Bernhard Steger

View all publications

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Normal Donkey Serum