Z-YVAD-FMK, Irreversible caspase-1 inhibitor (ab141388)
Key features and details
- Irreversible caspase-1 inhibitor. Cell-permeable.
- CAS Number: 210344-97-1
- Purity: > 98%
Soluble in DMSO to 20 mM
- Form / State: Lyophilized
- Source: Synthetic
製品の概要
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製品名
Z-YVAD-FMK, Irreversible caspase-1 inhibitor -
製品の詳細
Irreversible caspase-1 inhibitor. Cell-permeable. -
精製度
> 98% -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 1141 YVAD-FMK, Z-,. 1141-5 is the same size as the 5 mg size of ab141388.
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CAS 番号
210344-97-1 -
構造式
製品の特性
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分子量
630.70 -
配列
YVAD (Modifications: N-terminal benzyloxycarbonyl; C-terminal FMK; Asp-4 = Asp(OMe)) -
PubChem 登録番号
644196 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 20 mM
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使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CC1=CC=C(C=C1)O)NC(=O)OCC2=CC=CC=C2 -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab141388の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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Chemical Structure - Z-YVAD-FMK, Irreversible caspase-1 inhibitor (ab141388)2D chemical structure image of ab141388, Z-YVAD-FMK, Irreversible caspase-1 inhibitor
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Cellular activation - Z-YVAD-FMK, Irreversible caspase-1 inhibitor (ab141388)Image from Migita K, et al. Plos One, 9(5), e96703. Fig 3a,; doi: 10.1371/journal.pone.0096703Neutrophils were stimulated with SAA (5 µg/ml) in the presence or absence of Z-YVAD-FMK for 8 h. After stimulation, supernatants were analyzed for IL-1 Β production using ELISA. Values represent the mean ± SD of two independent experiments. *p<0.001 compared to SAA-stimulated neutrophils. -
Functional Studies - Z-YVAD-FMK, Irreversible caspase-1 inhibitor (ab141388)SHSY5Y cells were incubated at 37°C for 1h with vehicle control (0 μM) and different concentrations of Z-YVAD-FMK (ab141388). After this incubation 10 µM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic SHSY5Y cells correlates with an increase in Z-YVAD-FMK concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab37722 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development so
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (11)
ab141388 は 11 報の論文で使用されています。
- He S et al. PRRSV Infection Induces Gasdermin D-Driven Pyroptosis of Porcine Alveolar Macrophages through NLRP3 Inflammasome Activation. J Virol 96:e0212721 (2022). PubMed: 35758658
- Chen H et al. NLRP12 collaborates with NLRP3 and NLRC4 to promote pyroptosis inducing ganglion cell death of acute glaucoma. Mol Neurodegener 15:26 (2020). PubMed: 32295623
- Zhi X et al. NLRP3 inflammasome activation by Foot-and-mouth disease virus infection mainly induced by viral RNA and non-structural protein 2B. RNA Biol 17:335-349 (2020). PubMed: 31840571
- Buscetta M et al. Cigarette smoke inhibits the NLRP3 inflammasome and leads to caspase-1 activation via the TLR4-TRIF-caspase-8 axis in human macrophages. FASEB J 34:1819-1832 (2020). PubMed: 31914643
- Benyoucef A et al. CRISPR gene-engineered CYBBko THP-1 cell lines highlight the crucial role of NADPH-induced reactive oxygen species for regulating inflammasome activation. J Allergy Clin Immunol 145:1690-1693.e5 (2020). PubMed: 31954113
