Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor

• FuncS

Unknown

Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (AB120487)

HeLa cells were incubated at 37°C for 1h with vehicle control (0 μM) and different concentrations of Z-VAD(OMe)-FMK (ab120487). After this incubation 10 μM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic HeLa cells correlates with an increase in Z-VAD(OMe)-FMK concentration, as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab37722 at 1 μg /ml and ab8227 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution.

• FuncS

Lab

Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (AB120487)

Titration of the Caspase inhibitor Z-VAD(OMe)-FMK (ab120487) (duplicates; +/- SD).

• FuncS

Unknown

Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (AB120487)

Functional assays : Caspase 3 (active) Red Staining Kit (ab65617)

Caspase 3 activity in Jurkat cells (3 x10e5 cells) following 24 hour exposure to 2 μM Camptothecin (ab120115) with or without 50 μM caspase inhibitor Z-VAD(OMe)-FMK (ab120487). Background signal subtracted, duplicates; +/- SD.

• Chemical Structure

Lab

Chemical Structure - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (AB120487)

2D chemical structure image of ab120487, Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor