Y-27632 dihydrochloride, Rho kinase inhibitor (ab120129)
Key features and details
- Selective Rho kinase inhibitor
- CAS Number: 129830-38-2
- Soluble in water to 100 mM and in DMSO to 100mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Y-27632 dihydrochloride, Rho kinase inhibitor -
製品の詳細
Selective Rho kinase inhibitor -
生理活性の詳細
Y-27632 is a research tool with a wide variety of biological effects, mainly used in the field of stem cell research for maintenance and differentiation. Commonly used as 3D growth matrix component; also a component of brain organoid differentiation media.
How others have used Y-27632 in their research:
Maintenance of tissue culture of iPSCs; 10 μM Y-27632 for 24 hr; Montefiori et al (2018)
Reprogramming of breast cancer cells to induced progenitor-like cells (iPLs) ; 2 μM Y27632 ; Yuan et al (2018)
Differentiation - Y-27632 enables human embryonic stem cells to survive and differentiate into cortical and basal telencephalic progenitors; Watanabe et al (2007)
*Also available in simple stock solutions (ab144494) - add 1 ml of water to get an exact, ready-to-use concentration.
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CAS 番号
129830-38-2 -
構造式
製品の特性
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体系名
(R)-(+)-trans-4-(1-Aminoethyl)-N-(4-pyridyl)cyclohexanecarboxamide dihydrochloride -
分子量
320.26 -
分子式
C14H21N3O.2HCl -
PubChem 登録番号
9901617 -
保存方法
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 2 years. -
溶解性
Soluble in water to 100 mM and in DMSO to 100mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
Cl.Cl.O=C(Nc1ccncc1)[C@@H]2CC[C@H](CC2)[C@@H](C)N -
由来
Synthetic
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研究分野
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120129の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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Chemical Structure - Y-27632 dihydrochloride, Rho kinase inhibitor (ab120129)2D chemical structure image of ab120129, Y-27632 dihydrochloride, Rho kinase inhibitor
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Functional Studies - Y-27632 dihydrochloride, Rho kinase inhibitor (ab120129)Shiri D et al. PLoS One. 2012; 7(7): e41958. doi: 10.1371/journal.pone.0044400 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/A: Serum-starved human TM cells were incubated on chamber glass slides coated with recombinant human Gal8 in the presence of the ROCK inhibitor ab120129, at different concentrations. After 2 hr, cells were stained with rhodamine-labeled phalloidin, and cells with robust stress fibers were enumerated. Data are expressed as mean±SEM.
B: Cells were treated with an 120129 (Y27632) at 20 µM, stained with rhodamine-labeled phalloidin, and random fields were photographed. Note that cells treated with ab120129 are not spread and exhibit dendrite-like structures.
Credit: Shiri D et al. PLoS One. 2012; 7(7): e41958. doi: 10.1371/journal.pone.0044400
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Functional Studies - Y-27632 dihydrochloride, Rho kinase inhibitor (ab120129)ab46154 staining VEGF in HeLa cells treated with Y-27632 dihydrochloride (ab120129), by ICC/IF. Decrease in VEGF expression correlates with increased concentration of Y-27632 dihydrochloride, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120129 (Y-27632 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab46154 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (126)
ab120129 は 126 報の論文で使用されています。
- Mensah MA et al. Aberrant phase separation and nucleolar dysfunction in rare genetic diseases. Nature 614:564-571 (2023). PubMed: 36755093
- Schaafsma P et al. Role of immediate early genes in the development of salivary gland organoids in polyisocyanopeptide hydrogels. Front Mol Biosci 10:1100541 (2023). PubMed: 36818041
- Daskoulidou N et al. Complement receptor 1 is expressed on brain cells and in the human brain. Glia 71:1522-1535 (2023). PubMed: 36825534
- Shutova MS et al. Inflammation modulates intercellular adhesion and mechanotransduction in human epidermis via ROCK2. iScience 26:106195 (2023). PubMed: 36890793
- Yang H et al. Rescue of GM3 synthase deficiency by spatially controlled, rAAV-mediated ST3GAL5 delivery. JCI Insight 8:N/A (2023). PubMed: 37014712