Sodium butyrate, Histone deacetylase inhibitor (ab120948)
Key features and details
- Histone deacetylase inhibitor
- CAS Number: 156-54-7
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Sodium butyrate, Histone deacetylase inhibitor -
製品の詳細
Histone deacetylase inhibitor -
生理活性の詳細
Histone deacetylase inhibitor (IC50 values are 0.3, 0.4 and 0.3 mM for HDAC1, 2 and 7, respectively). Shows no inhibition of HDAC6 and 10. Anticancer activity. Crosses the blood-brain barrier.
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CAS 番号
156-54-7 -
構造式
製品の特性
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体系名
Sodium butanoate -
分子量
110.09 -
分子式
C4H7NaO2 -
PubChem 登録番号
5222465 -
保存方法
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
CCCC(=O)[O-].[Na+] -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120948の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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Western blot - Sodium butyrate, Histone deacetylase inhibitor (ab120948)
HeLa cells were incubated at 37°C for 6h with vehicle control (0 µM) and different concentrations of sodium butyrate (ab120948). Increased expression of histone H3 (acetyl K27) (ab4729) in HeLa cells correlates with an increase sodium butyrate concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab4729 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
HeLa cells were incubated at 37°C for 6h with vehicle control (0 µM) and different concentrations of sodium butyrate (ab120948). Increased expres
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Chemical Structure - Sodium butyrate, Histone deacetylase inhibitor (ab120948)2D chemical structure image of ab120948, Sodium butyrate, Histone deacetylase inhibitor -
Immunocytochemistry/ Immunofluorescence - Sodium butyrate, Histone deacetylase inhibitor (ab120948)ab4729 staining Histone H3 (acetyl K27) in HeLa cells. The cells were incubated with 10mM Sodium butyrate (ab120948) for 6 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab4729 at 0.5µg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with a anti-rabbit AlexaFluor®488 secondary antibody (ab150077) at 2 μg/ml (shown in green) and a goat anti-mouse AlexaFluor®594 (ab150120) at 2 μg/ml (shown in pseudo colour red). Nuclear DNA was labelled in blue with DAPI. Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (1)
ab120948 は 1 報の論文で使用されています。
- Bogacheva MS et al. Differences in definitive endoderm induction approaches using growth factors and small molecules. J Cell Physiol 233:3578-3589 (2018). PubMed: 29044512