(R,S)-3,5-DHPG, group I mGlu receptor agonist (ab120020)
Key features and details
- Group I mGlu receptor agonist
- CAS Number: 146255-66-5
- Purity: > 98%
Soluble in water to 10 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
(R,S)-3,5-DHPG, group I mGlu receptor agonist -
製品の詳細
Group I mGlu receptor agonist -
精製度
> 98% -
CAS 番号
146255-66-5 -
構造式
製品の特性
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体系名
2-amino-2-(3,5-dihydroxyphenyl)acetic acid
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分子量
183.16 -
分子式
C8H9NO4 -
PubChem 登録番号
108001 -
保存方法
Store at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes. -
溶解性
Soluble in water to 10 mM
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使用に関する注意
This compound is also unstable in alkaline solution when it rapidly forms a brown colouration it is therefore recommended that alkaline is not used to dissolve this compound.
We recommend stock solutions are made up in water, aliquoted immediately and stored at -20°C. They should be used within one week.Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
C1=C(C=C(C=C1O)O)C(C(=O)O)N -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120020の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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Chemical Structure - (R,S)-3,5-DHPG, group I mGlu receptor agonist (ab120020)2D chemical structure image of ab120020, (R,S)-3,5-DHPG, group I mGlu receptor agonist
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Immunocytochemistry/ Immunofluorescence - (R,S)-3,5-DHPG, group I mGlu receptor agonist (ab120020)ab17722 staining FMRP in SK-N-SH cells treated with (R,S)-3,5-DHPG (ab120020), by ICC/IF. Increase in FMRP expression correlates with increased concentration of (R,S)-3,5-DHPG, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120020 ((R,S)-3,5-DHPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (5)
ab120020 は 5 報の論文で使用されています。
- Bowling H et al. Altered steady state and activity-dependent de novo protein expression in fragile X syndrome. Nat Commun 10:1710 (2019). PubMed: 30979884
- Lee K et al. Autism-associated Shank3 mutations alter mGluR expression and mGluR-dependent but not NMDA receptor-dependent long-term depression. Synapse 73:e22097 (2019). PubMed: 30868621
- Li JQ et al. Regulation of increased glutamatergic input to spinal dorsal horn neurons by mGluR5 in diabetic neuropathic pain. J Neurochem 112:162-72 (2010). PubMed: 19840219
- Roloff AM et al. Homer 1a gates the induction mechanism for endocannabinoid-mediated synaptic plasticity. J Neurosci 30:3072-81 (2010). PubMed: 20181604
- Musante V et al. Presynaptic mGlu1 and mGlu5 autoreceptors facilitate glutamate exocytosis from mouse cortical nerve endings. Neuropharmacology 55:474-82 (2008). PubMed: 18625255