Resorufin ß-D-galactopyranoside

Key facts

CAS番号

95079-19-9

精製度

>95%

製品の状態

Solid

form

分子量

375.3 Da

分子式

C₁₈H₁₇NO₈

PubChem

125273

由来

Synthetic

溶解性

Soluble in DMSO to 50 mM

化学名

Resorufin galactopyranoside

生物学的記述

Resorufin β-D-galactopyranoside requires only a single-step hydrolysis reaction to attain full fluorescence making it especially useful for sensitive enzyme measurements in applications such as ELISAs or high-content screening systems. Suitable for continuous measurement of enzyme activity at Ex/Em=573/585 nm. Can quantitate beta-galactosidaseactivity in single yeast cells via flow cytometry.

Canonical smiles

C1=CC2=C(C=C1OC3C(C(C(C(O3)CO)O)O)O)OC4=CC(=O)C=CC4=N2

Isomeric smiles

C1=CC2=C(C=C1O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)OC4=CC(=O)C=CC4=N2

InChi

InChI=1S/C18H17NO8/c20-7-14-15(22)16(23)17(24)18(27-14)25-9-2-4-11-13(6-9)26-12-5-8(21)1-3-10(12)19-11/h1-6,14-18,20,22-24H,7H2/t14-,15+,16+,17-,18-/m1/s1

InChiKey

QULZFZMEBOATFS-DISONHOPSA-N

IUPAC名

7-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyphenoxazin-3-one

励起波長/蛍光波長

Ex: 573nm, Em: 585nm