PPADS, P2 purinergic receptor antagonist (ab120009)
Key features and details
- P2 purinergic receptor antagonist
- CAS Number: 149017-66-3
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
PPADS, P2 purinergic receptor antagonist -
製品の詳細
P2 purinergic receptor antagonist -
生理活性の詳細
P2 purinergic receptor antagonist.
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CAS 番号
149017-66-3 -
構造式
製品の特性
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体系名
4-[[4-Formyl-5-hydroxy-6-methyl-3-[(phosphonooxy)methyl]-2-pyridinyl]azo]-1,3-benzenedisulfonic acid tetrasodium salt -
分子量
599.30 -
分子式
C14H10N3Na4O12PS2 -
PubChem 登録番号
4880 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
O=S(=O)(O[Na])c2ccc(/N=N/c1nc(C)c(O)c(C=O)c1COP(=O)(O[Na])O[Na])c(c2)S(=O)(=O)O[Na] -
由来
Synthetic
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研究分野
画像
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Chemical Structure - PPADS, P2 purinergic receptor antagonist (ab120009)2D chemical structure image of ab120009, PPADS, P2 purinergic receptor antagonist
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Cellular activation - PPADS, P2 purinergic receptor antagonist (ab120009)Image from Tchernookova B, et al. Plos One, 13(2), e0190893. Fig 2c,; doi: 10.1371/journal.pone.0190893Inhibition by suramin and PPADS significantly reduces the ATP-induced increase in extracellular H+ flux from isolated Müller cells.
Mean responses to 10 µM ATP with or without suramin and PPADS in the bath; N = 7, error bars represent SEMs. -
Functional Studies - PPADS, P2 purinergic receptor antagonist (ab120009)Image from Gómez-Gonzalo M et al., PLoS Biol. 2010;8(4):e1000352. Fig 2.; doi: 10.1371/journal.pbio.1000352. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Astrocyte Ca2+ signal inhibition does not affect interictal discharges. (A–D) Mean percentage of astrocytes activated by the ictal discharges (A), mean duration (B) and frequency (C) of the ictal discharge, and mean frequency of interictal discharges (D) under different experimental conditions in EC slice preparations. Controls (n=16), MPEP (ab120008) (n=7), PPADS (ab120009) (n=9), and MPEP+PPADS (n=3). A single asterisk (*) indicates p<0.05; double asterisks (**), p<0.01. -
Functional Studies - PPADS, P2 purinergic receptor antagonist (ab120009)Image from Gómez-Gonzalo M et al., PLoS Biol. 2010;8(4):e1000352. Fig 6(A).; doi: 10.1371/journal.pbio.1000352.Ca2+ signal from a field A neuron, a field B neuron, and field A astrocytes in response to repetitive episodes of NMDA stimulation (black arrowheads). The NMDA stimulation that evoked an ictal discharge became ineffective after blocking the astrocyte response by bath perfusion with MPEP (ab120008) and PPADS (ab120009). An ictal discharge could be recovered by increasing the number of NMDA puffs (white arrowheads). A double NMDA pulse evoked both astrocyte activation and the ictal discharge after inhibitor washout.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (10)
ab120009 は 10 報の論文で使用されています。
- Seto C et al. Influence of gap junctions upon Ca2+ propagation from stimulated keratinocytes to DRG neurons. Biophys Physicobiol 19:e190041 (2022). PubMed: 36349331
- Pulukool SK et al. Elevated dimethylarginine, ATP, cytokines, metabolic remodeling involving tryptophan metabolism and potential microglial inflammation characterize primary open angle glaucoma. Sci Rep 11:9766 (2021). PubMed: 33963197
- Bhagavatham SKS et al. Adenosine deaminase modulates metabolic remodeling and orchestrates joint destruction in rheumatoid arthritis. Sci Rep 11:15129 (2021). PubMed: 34301999
- Su WF et al. Overexpression of P2X4 receptor in Schwann cells promotes motor and sensory functional recovery and remyelination via BDNF secretion after nerve injury. Glia 67:78-90 (2019). PubMed: 30306657
- Tchernookova BK et al. Activation of retinal glial (Müller) cells by extracellular ATP induces pronounced increases in extracellular H+ flux. PLoS One 13:e0190893 (2018). PubMed: 29466379
- Ferreira-Neto HC et al. ATP stimulates rat hypothalamic sympathetic neurons by enhancing AMPA receptor-mediated currents. J Neurophysiol 114:159-69 (2015). PubMed: 25904713
- Heo SJ et al. Biophysical Regulation of Chromatin Architecture Instills a Mechanical Memory in Mesenchymal Stem Cells. Sci Rep 5:16895 (2015). PubMed: 26592929
- Kovacs FE et al. Purine receptor-mediated endocannabinoid production and retrograde synaptic signalling in the cerebellar cortex. Br J Pharmacol 162:974-88 (2011). PubMed: 21054344
- Toulme E et al. P2X4 receptors in activated C8-B4 cells of cerebellar microglial origin. J Gen Physiol 135:333-53 (2010). PubMed: 20231374
- Gómez-Gonzalo M et al. An excitatory loop with astrocytes contributes to drive neurons to seizure threshold. PLoS Biol 8:e1000352 (2010). PubMed: 20405049
