AB141032

PMSF, protease inhibitor

Name: PMSF, protease inhibitor (ab141032) | Abcam
Brand: Abcam Limited
SKU: AB141032
Price: 10000 JPY
Availability: InStock

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(14 Publications)

MW 174.19 Da, Purity >98%. Non-specific protease inhibitor; binds key active site serine residue in serine proteases, acetylcholinesterases, and thiol proteases. Suitable for use in vitro; in the preparation of cell lysates, and in vivo where it has been shown to block anandamide (ab120429) metabolism. Able to rapidly cross the BBB.

2 Images

Functional Studies (Act) - PMSF, protease inhibitor (AB141032)

FuncS (Act)

Unknown

Functional Studies (Act) - PMSF, protease inhibitor (AB141032)

Analysis of protein expression level in the sclera. The scleral tissue was homogenized and solubilized in ice-cold PBS containing PMSF protease inhibitor. Lumican and collagen type I were both overexpressed at the protein level in the transgenic sclera. GAPDH was used as a control. Lane 1 to lane 5, transgenic mice. Lane 6 to lane 9, wild-type mice.

Image from Song Y, et al. Plos One, 11(10), e0163165. Fig 5b,; doi: 10.1371/journal.pone.0163165

Chemical Structure - PMSF, protease inhibitor (AB141032)

Chemical Structure

Lab

Chemical Structure - PMSF, protease inhibitor (AB141032)

2D chemical structure image of ab141032, PMSF, protease inhibitor

Key facts

CAS番号

329-98-6

精製度

>98%

製品の状態

Solid

form

分子量

174.19 Da

分子式

C7H7FO2S

PubChem

4784

由来

Synthetic

溶解性

Insoluble in H2O

≥28.3 mg/mL in EtOH

≥17.4 mg/mL in DMSO

Insoluble in water

?17.4 mg/mL in DMSO

?28.3 mg/mL in EtOH

化学名

Phenylmethylsulfonyl fluoride

生物学的記述

Non-specific protease inhibitor; binds key active site serine residue in serine proteases, acetylcholinesterases, and thiol proteases. Suitable for use in vitro; in the preparation of cell lysates, and in vivo where it has been shown to block anandamide (ab120429) metabolism. Able to rapidly cross the BBB.

Canonical smiles

C1=CC=C(C=C1)CS(=O)(=O)F

InChi

InChI=1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2

InChiKey

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

IUPAC名

phenylmethanesulfonyl fluoride

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製品の詳細

Unstable in water

Storage instructions: Hygroscopic, Under inert atmosphere.

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出荷温度及び保存条件

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

Need more advice on solubility, usage and handling? Please visit our our product support page for more details.

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Toxic, refer to SDS for further information.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:126 PubMed39988592

2025

Glycolysis reprogramming in CAFs promotes oxaliplatin resistance in pancreatic cancer through circABCC4 mediated PKM2 nuclear translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Rihua He,Chonghui Hu,Yuan Yuan,Tingting Li,Qing Tian,Tianhao Huang,Qing Lin,Shangyou Zheng,Chujie Chen,Zhiqiang Fu,Rufu Chen

International journal of molecular sciences 25: PubMed39596535

2024

Astrocyte Dysfunction Reflected in Ischemia-Induced Astrocyte-Derived Extracellular Vesicles: A Pilot Study on Acute Ischemic Stroke Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Timea Forró,Doina Ramona Manu,Lucian Barbu-Tudoran,Rodica Bălașa

International journal of molecular sciences 25: PubMed38891912

2024

GFAP as Astrocyte-Derived Extracellular Vesicle Cargo in Acute Ischemic Stroke Patients-A Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

Timea Forró,Doina Ramona Manu,Ovidiu-Lucian Băjenaru,Rodica Bălașa

STAR protocols 5:103094 PubMed38796847

2024

Protocol for inducing severe Scn2a insufficiency in mice by intracerebroventricular antisense oligonucleotide injection.

Applications

Unspecified application

Species

Unspecified reactive species

Melody Li,Bernd Kuhn

Frontiers in cellular and infection microbiology 14:1374659 PubMed38524184

2024

infection-induced host cellular DNA damage is strain-dependent and leads to the activation of the ATM-dependent homologous recombination pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lisbeth Rojas-Barón,Carlos Hermosilla,Anja Taubert,Zahady D Velásquez

Frontiers in microbiology 15:1336267 PubMed38450167

2024

Me49 and NED strains arrest host cell cycle progression and alter chromosome segregation in a strain-independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Lisbeth Rojas-Barón,Carlos Hermosilla,Anja Taubert,Zahady D Velásquez

Pflugers Archiv : European journal of physiology 476:939-948 PubMed38446167

2024

Locally applied heat stress during exercise training may promote adaptations to mitochondrial enzyme activities in skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Ed Maunder,Andrew King,Jeffrey A Rothschild,Matthew J Brick,Warren B Leigh,Christopher P Hedges,Troy L Merry,Andrew E Kilding

iScience 27:109003 PubMed38327792

2024

A single cell atlas of circulating immune cells involved in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Liao,Wei Fan,Na Li,Ruonan Li,Xiaotang Wang,Jiangyi Liu,Hong Wang,Shengping Hou

iScience 26:108138 PubMed37876801

2023

Scn2a insufficiency alters spontaneous neuronal Ca activity in somatosensory cortex during wakefulness.

Applications

Unspecified application

Species

Unspecified reactive species

Melody Li,Mohamed Eltabbal,Hoang-Dai Tran,Bernd Kuhn

PeerJ 11:e16060 PubMed37790613

2023

Fluorofenidone alleviates liver fibrosis by inhibiting hepatic stellate cell autophagy the TGF-β1/Smad pathway: implications for liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiongqun Peng,Huixiang Yang,Lijian Tao,Jingni Xiao,Ya Zeng,Yueming Shen,Xueke Yu,Fei Zhu,Jiao Qin

View all publications

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PMSF, protease inhibitor