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NMDA, excitotoxic amino acid (ab120052)

Submit a review Q&A (1)References (18)
Chemical Structure - NMDA, excitotoxic amino acid (ab120052)
  • Functional Studies - NMDA, excitotoxic amino acid (ab120052)
  • Immunocytochemistry/ Immunofluorescence - NMDA, excitotoxic amino acid (ab120052)

Key features and details

  • Excitotoxic amino acid
  • CAS Number: 6384-92-5
  • Soluble in water to 100 mM
  • Form / State: Solid
  • Source: Synthetic

製品の概要

  • 製品名

    NMDA, excitotoxic amino acid

  • 製品の詳細

    Excitotoxic amino acid

  • 生理活性の詳細

    Excitotoxic amino acid. Prototypic agonist at the ionotropic NMDA glutamate receptor which is involved in long-term potentiation, ischemia, and epilepsy. Also available in Kit: Ionotropic agonists (ab120323).

    Also available in simple stock solutions (ab146698) - add 1 ml of water to get an exact, ready-to-use concentration.

  • CAS 番号

    6384-92-5

  • 構造式

    Chemical Structure

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab120052の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Functional Studies
Use at an assay dependent concentration.
特記事項
Functional Studies
Use at an assay dependent concentration.

画像

  • Chemical Structure - NMDA, excitotoxic amino acid (ab120052)
    Chemical Structure - NMDA, excitotoxic amino acid (ab120052)
    2D chemical structure image of ab120052, NMDA, excitotoxic amino acid
  • Functional Studies - NMDA, excitotoxic amino acid (ab120052)
    Functional Studies - NMDA, excitotoxic amino acid (ab120052)Sims et al PLoS One. 2013;8(1):e53814. doi: 10.1371/journal.pone.0053814. Epub 2013 Jan 11. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Release of adenosine by depolarisation and agonists.

    (Panel c) NMDA application also evoked neuronal depolarisation and firing accompanied by subsequent release of adenosine and inosine.

  • Immunocytochemistry/ Immunofluorescence - NMDA, excitotoxic amino acid (ab120052)
    Immunocytochemistry/ Immunofluorescence - NMDA, excitotoxic amino acid (ab120052)
    ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with NMDA (ab120052), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of NMDA, as described in literature.
    The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120052 (NMDA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (18)

ab120052 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab120052 は 18 報の論文で使用されています。

  • Xu D  et al. Overexpressing NeuroD1 reprograms Müller cells into various types of retinal neurons. Neural Regen Res 18:1124-1131 (2023). PubMed: 36255002
  • Špirková A  et al. Glutamate can act as a signaling molecule in mouse preimplantation embryos†. Biol Reprod 107:916-927 (2022). PubMed: 35746896
  • Bowers MS  et al. NYX-2925 induces metabotropic N-methyl-d-aspartate receptor (NMDAR) signaling that enhances synaptic NMDAR and a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor. J Neurochem 152:523-541 (2020). PubMed: 31376158
  • Kiragasi B  et al. The auxiliary glutamate receptor subunit dSol-1 promotes presynaptic neurotransmitter release and homeostatic potentiation. Proc Natl Acad Sci U S A 117:25830-25839 (2020). PubMed: 32973097
  • Girling KD  et al. Activation of caspase-6 and cleavage of caspase-6 substrates is an early event in NMDA receptor-mediated excitotoxicity. J Neurosci Res 96:391-406 (2018). PubMed: 29193273
View all Publications for this product

レビューと Q&A

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Question

To whom it may concern, We use NMDA to induce a cognitive deficit in an passive avoidance task in rat. I have used NMDA from sigma-aldrich (product code XXXX) before which is in a free aminoacid form. Now we are trying your NMDA which is in a hemihydrate form (ab120052) and we have some problems. First it is hard to disollute (15 min in ultrasonic bath, 42 mg/1ml water) in comparsion to the sigmas NMDA. Second, the rats behave different. Abcam NMDA seems to be stronger and some rats have epileptoformic seizures after systemic administration (42 mg/kg i.p.). We have observed also some mortality, approx. 10 %. With Sigma NMDA we did not observe any seizures or mortality. Do you have any idea why it is happening ? May I kindly ask you for NMR spectra of this batch ? with best regards

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Answer

Thank you for contacting us.
I am sorry to hear you are not observing the expected results with the reagent supplied.
It is possible that ab120052 acts more potently due to it having a higher purity when compared to Sigma’s. I have attached the NMP spectra for ab120052. If you had Sigma’s NMR spectra for this product, we would be happy to compare it against ours to determine if this is the case.
It is also worth considering that variations in the strength of the epileptoformic seizures can occur due to variations in batches of rats.
We would also recommend using the batch specific molecular weight when calculating doses.
As a final note, variations in seizure strength could occur if the solvent/saline solution is not fresh or contaminated – this should be checked before carrying out experimental procedures.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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