MiTMAB™, dynamin I and dynamin II inhibitor (ab120466)
Key features and details
- Cell-permeable dynamin I and dynamin II inhibitor
- CAS Number: 1119-97-7
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
-
製品名
MiTMAB™, dynamin I and dynamin II inhibitor -
製品の詳細
Cell-permeable dynamin I and dynamin II inhibitor -
生理活性の詳細
Cell-permeable dynamin I and dynamin II inhibitor (IC50 values are 3.1 and 8.4 µM for inhibition of dynamin I and dynamin II GTPase, respectively). Targets the pleckstrin homology (PH) (lipid binding) domain. Competitive with phospholipid and non-competitive with GTP. Inhibits receptor-mediated and synaptic vesicle endocytosis (IC50 values are 19.9 and 2.2 µM, respectively). Inhibits cancer cell growth.
-
特記事項
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. MiTMAB™ and OcTMAB™ are trademarks of Children's Medical Research Institute and Newcastle Innovation Ltd. -
CAS 番号
1119-97-7 -
構造式
製品の特性
-
体系名
Tetradecyltrimethylammonium bromide -
分子量
336.40 -
分子式
C17H38BrN -
PubChem 登録番号
14250 -
保存方法
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES 線形表記
[Br-].C[N+](C)(C)CCCCCCCCCCCCCC -
由来
Synthetic
-
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120466の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
画像
-
Chemical Structure - MiTMAB™, dynamin I and dynamin II inhibitor (ab120466)2D chemical structure image of ab120466, MiTMAB™, dynamin I and dynamin II inhibitor
-
Immunocytochemistry/ Immunofluorescence - MiTMAB™, dynamin I and dynamin II inhibitor (ab120466)ab66705 staining PAI1 in HeLa cells treated with MiTMAB™ (ab120466), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of MiTMAB™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120466 (MiTMAB™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (10)
ab120466 は 10 報の論文で使用されています。
- Wang D et al. ATP Consumption Is Coupled with Endocytosis in Exudated Neutrophils. Int J Mol Sci 24:N/A (2023). PubMed: 37240384
- Štimac I et al. Dynamin Inhibitors Prevent the Establishment of the Cytomegalovirus Assembly Compartment in the Early Phase of Infection. Life (Basel) 11:N/A (2021). PubMed: 34575026
- Lynn KS et al. Asymmetric distribution of dynamin-2 and ß-catenin relative to tight junction spikes in alveolar epithelial cells. Tissue Barriers N/A:1929786 (2021). PubMed: 34107845
- Wang D et al. ERK is involved in the differentiation and function of dimethyl sulfoxide-induced HL-60 neutrophil-like cells, which mimic inflammatory neutrophils. Int Immunopharmacol 84:106510 (2020). PubMed: 32361568
- Metwally H et al. Noncanonical STAT1 phosphorylation expands its transcriptional activity into promoting LPS-induced IL-6 and IL-12p40 production. Sci Signal 13:N/A (2020). PubMed: 32209697
- Aoto M et al. Transferrin receptor 1 is required for enucleation of mouse erythroblasts during terminal differentiation. FEBS Open Bio 9:291-303 (2019). PubMed: 30761254
- Pfanzelter J et al. Septins suppress the release of vaccinia virus from infected cells. J Cell Biol 217:2911-2929 (2018). PubMed: 29921601
- Grassart A et al. Actin and dynamin2 dynamics and interplay during clathrin-mediated endocytosis. J Cell Biol 205:721-35 (2014). PubMed: 24891602
- Fiore VF et al. Dynamic catch of a Thy-1-a5ß1+syndecan-4 trimolecular complex. Nat Commun 5:4886 (2014). PubMed: 25216363
- Nimitvilai S et al. Reversal of dopamine D2 agonist-induced inhibition of ventral tegmental area neurons by Gq-linked neurotransmitters is dependent on protein kinase C, G protein-coupled receptor kinase, and dynamin. J Pharmacol Exp Ther 344:253-63 (2013). PubMed: 23019137