GANT 61, GLI1 and 2 inhibitor (ab120904)
Key features and details
- Selective GLI1 and 2 inhibitor
- CAS Number: 500579-04-4
- Purity: > 98%
- Soluble in DMSO to 50 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
GANT 61, GLI1 and 2 inhibitor -
製品の詳細
Selective GLI1 and 2 inhibitor -
別名
- NSC 136476
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生理活性の詳細
Selective GLI1 and 2 inhibitor (IC50 = 5 μM). Blocks the hedgehog/smoothened signaling pathway. Anticancer activity in vitro and in vivo. -
精製度
> 98% -
CAS 番号
500579-04-4 -
構造式
製品の特性
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体系名
2,2'-[[Dihydro-2-(4-pyridinyl)-1,3(2H,4H)-pyrimidinediyl]bis(methylene)]bis(N,N-dimethylbenzenamine) -
分子量
429.61 -
分子式
C27H35N5 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 50 mM and in ethanol to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
CN(C)C1=CC=CC=C1CN2CCCN(CC3=CC=CC=C3N(C)C)C2C4=CC=NC=C4 -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120904の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
画像
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Chemical Structure - GANT 61, GLI1 and 2 inhibitor (ab120904)2D chemical structure image of ab120904, GANT 61, GLI1 and 2 inhibitor
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Functional Studies - GANT 61, GLI1 and 2 inhibitor (ab120904)ab11169 staining MDC1 in HCT116 cells treated with GANT 61 (ab120904), by ICC/IF. Increase in MDC1 expression correlates with increased concentration of GANT 61, as described in literature.
The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120904 (GANT 61) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11169 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab120904 は 6 報の論文で使用されています。
- Zeng C et al. Hedgehog signaling activation required for glypican-6-mediated regulation of invasion, migration, and epithelial-mesenchymal transition of gastric cancer cells. Biosci Rep 40:N/A (2020). PubMed: 32478377
- Qi H et al. Inactivation of Nell-1 in Chondrocytes Significantly Impedes Appendicular Skeletogenesis. J Bone Miner Res 34:533-546 (2019). PubMed: 30352124
- Dong H et al. GLI1 activation by non-classical pathway integrin avß3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis. Cell Death Dis 10:574 (2019). PubMed: 31366904
- Shao JB et al. The mechanism of epithelial-mesenchymal transition induced by TGF-ß1 in neuroblastoma cells. Int J Oncol 50:1623-1633 (2017). PubMed: 28393230
- Zhang C et al. Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling. Oncotarget 7:86134-86147 (2016). Blocking ; Human . PubMed: 27863385
- Xu Y et al. Inhibition of the Hedgehog pathway induces autophagy in pancreatic ductal adenocarcinoma cells. Oncol Rep 31:707-12 (2014). PubMed: 24297612
