Forskolin, Adenylyl cyclase activator (ab120058)
Key features and details
- Adenylyl cyclase activator
- CAS Number: 66575-29-9
- Soluble in ethanol to 10 mM and in DMSO to 25 mM
- Form / State: Solid
- Source: Coleus forskohlii
製品の概要
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製品名
Forskolin, Adenylyl cyclase activator -
製品の詳細
Adenylyl cyclase activator -
生理活性の詳細
Cell-permeable activator of adenylyl cyclase. Interacts directly with the catalytic subunit of the enzyme to increase intracellular cAMP levels. Positive inotropic, platelet antiaggregatory and antihypertensive actions in vivo. 3D Growth matrix component used in liver organoid generation.
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CAS 番号
66575-29-9 -
構造式
製品の特性
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体系名
(3R,4aR,5S,6S,6aS,10S,10aR,10bS)-Dodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-3-vinyl-1H-benzo[f]chromen-5-yl acetate -
分子量
410.50 -
分子式
C22H34O7 -
PubChem 登録番号
47936 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in ethanol to 10 mM and in DMSO to 25 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
CC(=O)O[C@H]2[C@@H](O)[C@@H]1[C@](C)([C@@H](O)CCC1(C)C)[C@@]3(O)C(=O)C[C@](C)(C=C)O[C@]23C -
由来
Coleus forskohlii
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研究分野
- Biochemicals
- Pharmacology
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
- Biochemicals
- Research Area
- Heart disease
- Signaling
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Pain & inflammation
- Signaling
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Obesity
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Respiratory disease
- Signaling
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
画像
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Chemical Structure - Forskolin, Adenylyl cyclase activator (ab120058)2D chemical structure image of ab120058, Forskolin, Adenylyl cyclase activator
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Functional Studies - Forskolin, Adenylyl cyclase activator (ab120058)Gambino et al PLoS One. 2010 Jul 2;5(7):e11399. doi: 10.1371/journal.pone.0011399. Fig S4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Effect of AC activation onto adult Mecp2X/Y and Mecp2308/Y Cortico-LA synapses.
A: A 10 minute application of the AC activator Forskolin (FSK, 50 µM) potentiates the Cortico-LA EPSC in young WT animals.
B: The same protocol is inefficient in adult WT mice (B1) and Mecp2308/Y mice (B2).
C: Summary plot of similar pharmacological experiments in WT and KO mice.
***: P<0.001.
Number of recorded cells is indicated.
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Functional Studies - Forskolin, Adenylyl cyclase activator (ab120058)ab2541 staining XIAP in MCF7 cells treated with forskolin (ab120058), by ICC/IF. Increase in XIAP expression correlates with increased concentration of forskolin, as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120058 (forskolin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2541 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. -
Functional Studies - Forskolin, Adenylyl cyclase activator (ab120058)1e7 3T3-L1 cells were treated for 15 minutes with 1 µM dexamethasone (ab120743) and 1 µg x mL-1 insulin (ab123768) with the addition of 50 µM forskolin (ab120058), 0.5 mM IBMX (ab120840) or 0.1 mM 8-CPT-cAMP (ab120424), extracted according to the protocol. Lysates were diluted 50-fold, and tested in duplicates (+/-SD).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (47)
ab120058 は 47 報の論文で使用されています。
- Mou X et al. An ultrathin membrane mediates tissue-specific morphogenesis and barrier function in a human kidney chip. Sci Adv 10:eadn2689 (2024). PubMed: 38838141
- Chen S et al. PAQR8 promotes breast cancer recurrence and confers resistance to multiple therapies. Breast Cancer Res 25:1 (2023). PubMed: 36597146
- Fernando SJA et al. Evidence that RXFP4 is located in enterochromaffin cells and can regulate production and release of serotonin. Biosci Rep 43:N/A (2023). PubMed: 36947541
- Xu X et al. Single-cell transcriptomic analysis uncovers the origin and intratumoral heterogeneity of parotid pleomorphic adenoma. Int J Oral Sci 15:38 (2023). PubMed: 37679344
- Sturiale V et al. Cell Cycle Reactivation, at the Start of Neurodegeneration, Induced by Forskolin and Aniline in Differentiated Neuroblastoma Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37762676