Fluo-8 AM, green fluorescent calcium binding dye (ab142773)
Key features and details
- Medium affinity green fluorescent calcium binding dye. Cell-permeable.
- CAS Number: 1345980-40-6
Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Fluo-8 AM, green fluorescent calcium binding dye -
製品の詳細
Medium affinity green fluorescent calcium binding dye. Cell-permeable. -
特記事項
Fluo-8® AM Working Solution: On the day of the experiment, either dissolve Fluo-8® AM in DMSO or thaw an aliquot of the indicator stock solution to room temperature. Prepare a dye working solution of 2 to 20 µM in a buffer of your choice (e.g., Hanks and Hepes buffer) with 0.04% Pluronic® F-127. For most cell lines, Fluo-8® AM at a final concentration of 4-5 μM is recommended. The exact concentration of indicators required for cell loading must be determined empirically.
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CAS 番号
1345980-40-6 -
構造式
製品の特性
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励起波長
490nm -
蛍光波長
520nm -
体系名
Bis(acetoxymethyl) 2,2'-((4-(6-(acetoxymethoxy)-3-oxo-3H-xanthen-9-yl)-2-(2-(bis(2-acetoxymethoxy)-2-oxoethyl)amino)phenoxy)ethoxy)phenyl)azanediyl)diacetate
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分子量
1046.94 -
分子式
C50H50N2O23 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO
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使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
For more information on AM esters please visit our AM esters FAQ page.Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c5ccccc5OCCOc1cc(ccc1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=2c4ccc(cc4OC3=CC(=O)C=CC=23)OCOC(C)=O -
由来
Synthetic
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研究分野
画像
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Chemical Structure - Fluo-8 AM, green fluorescent calcium binding dye (ab142773)2D chemical structure image of ab142773, Fluo-8 AM, green fluorescent calcium binding dye
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Functional Studies - Fluo-8 AM, green fluorescent calcium binding dye (ab142773)Cui et al PLoS One. 2013;8(3):e59921. doi: 10.1371/journal.pone.0059921. Epub 2013 Mar 20. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/FFA treatments increase Ca2+ influx to induce ERS in β-TC3 cells.
β-TC3 cells were incubated with FFA or BSA for 16 h, and then stimulated with 4 µM thapsigargin for 20 min to activate store-operated Ca2+ entry. Fluorescence densities of Ca2+ change were monitored in Fluo-8/AM-loaded β-TC3 cells after FFA or BSA treatments.
(From Figure 2B of Cui et al)
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Fluorescent Cell Imaging - Fluo-8 AM, green fluorescent calcium binding dye (ab142773)U2OS cells were seeded overnight at 40,000 cells per 100 µL per well in a 96-well black wall/clear bottom costar plate. The growth medium was removed, and the cells were incubated with 100 µl of 4 µM Fluo-4 AM or Fluo-8® AM in HHBS at 37 °C, 5% CO2 incubator for 1 hour. The cells were washed twice with 200 µl HHBS, then imaged with a fluorescence microscope using FITC channel.
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Functional Studies - Fluo-8 AM, green fluorescent calcium binding dye (ab142773)Fluo-8 vs Fluo-4 sensitivity to calcium release in HEK-293 cells induced by Carbachol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (51)
ab142773 は 51 報の論文で使用されています。
- Erbacher C et al. Interaction of human keratinocytes and nerve fiber terminals at the neuro-cutaneous unit. Elife 13:N/A (2024). PubMed: 38225894
- Chechekhin V et al. Alpha1A- and Beta3-Adrenoceptors Interplay in Adipose Multipotent Mesenchymal Stromal Cells: A Novel Mechanism of Obesity-Driven Hypertension. Cells 12:N/A (2023). PubMed: 36831252
- Wang M et al. Axin2 coupled excessive Wnt-glycolysis signaling mediates social defect in autism spectrum disorders. EMBO Mol Med 15:e17101 (2023). PubMed: 37078424
- You W et al. Stressed neuronal cells can recover from profound membrane blebbing, nuclear condensation and mitochondrial fragmentation, but not from cytochrome c release. Sci Rep 13:11045 (2023). PubMed: 37422517
- Ji L et al. Secretogranin III Selectively Promotes Vascular Leakage in the Deep Vascular Plexus of Diabetic Retinopathy. Int J Mol Sci 24:N/A (2023). PubMed: 37445707