AB120223

FK506 (Tacrolimus), Immunosuppressant

Name: FK506 (Tacrolimus), Immunosuppressant (ab120223) | Abcam
Brand: Abcam Limited
SKU: AB120223
Price: 29000 JPY
Availability: InStock

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(13 Publications)

MW 804 Da, Purity >98%. Immunosuppressant. Complexes with FKBP-12 to inhibit calcineurin. Achieve your results faster with highly validated, pure and trusted compounds.

別名を表示する

12 kDa FK506-binding protein, 12 kDa FKBP, Calstabin 1, FK506 binding protein 1, FK506 binding protein 12, FK506 binding protein 1A (12kD), FK506 binding protein 1A 12kDa, FK506 binding protein T cell 12 kD, FK506-binding protein 1A, FKB1A_HUMAN, FKBP-1A, FKBP1, FKBP12 Exip3, FKBP12C, Immunophilin FKBP12, PKC12, PKCI2, PPIase, PPIase FKBP1A, Peptidyl-prolyl cis-trans isomerase FKBP1A, Protein kinase C inhibitor 2, Rotamase, peptidyl-prolyl cis-trans isomerase

2 Images

Immunocytochemistry/ Immunofluorescence - FK506 (Tacrolimus), Immunosuppressant (AB120223)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - FK506 (Tacrolimus), Immunosuppressant (AB120223)

ab32518 staining IkBα/β in RAW 264.7 cells treated with FK506 (ab120223), by ICC/IF. Decrease in IkBα/β expression correlates with increased concentration of FK506, as described in literature.
The cells were incubated at 37°C for 3h in media containing different concentrations of ab120223 (FK506) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32518 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Chemical Structure - FK506 (Tacrolimus), Immunosuppressant (AB120223)

Chemical Structure

Lab

Chemical Structure - FK506 (Tacrolimus), Immunosuppressant (AB120223)

2D chemical structure image of ab120223, FK506 (Tacrolimus), Immunosuppressant

Key facts

CAS番号

104987-11-3

精製度

>98%

製品の状態

Solid

form

作製由来

Streptomyces tsukubaensis

分子量

804 Da

分子式

C44H69NO12

PubChem

445643

由来

Native

溶解性

Soluble in DMSO to 100 mM

化学名

Tacrolimus

生物学的記述

Immunosuppressant. Complexes with FKBP-12 to inhibit calcineurin.

Canonical smiles

CC1CC(C2C(CC(C(O2)(C(=O)C(=O)N3CCCCC3C(=O)OC(C(C(CC(=O)C(C=C(C1)C)CC=C)O)C)C(=CC4CCC(C(C4)OC)O)C)O)C)OC)OC

Isomeric smiles

C[C@@H]1C[C@@H]([C@@H]2[C@H](C[C@H]([C@@](O2)(C(=O)C(=O)N3CCCC[C@H]3C(=O)O[C@@H]([C@@H]([C@H](CC(=O)[C@@H](/C=C(/C1)\C)CC=C)O)C)/C(=C/[C@@H]4CC[C@H]([C@@H](C4)OC)O)/C)O)C)OC)OC

InChi

InChI=1S/C44H69NO12/c1-10-13-31-19-25(2)18-26(3)20-37(54-8)40-38(55-9)22-28(5)44(52,57-40)41(49)42(50)45-17-12-11-14-32(45)43(51)56-39(29(6)34(47)24-35(31)48)27(4)21-30-15-16-33(46)36(23-30)53-7/h10,19,21,26,28-34,36-40,46-47,52H,1,11-18,20,22-24H2,2-9H3/b25-19+,27-21+/t26-,28+,29+,30-,31+,32-,33+,34-,36+,37-,38-,39+,40+,44+/m0/s1

InChiKey

QJJXYPPXXYFBGM-LFZNUXCKSA-N

IUPAC名

(1R,9S,12S,13R,14S,17R,18E,21S,23S,24R,25S,27R)-1,14-dihydroxy-12-[(E)-1-[(1R,3R,4R)-4-hydroxy-3-methoxycyclohexyl]prop-1-en-2-yl]-23,25-dimethoxy-13,19,21,27-tetramethyl-17-prop-2-enyl-11,28-dioxa-4-azatricyclo[22.3.1.04,9]octacos-18-ene-2,3,10,16-tetrone

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出荷温度及び保存条件

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

Store under desiccating conditions|The product can be stored for up to 12 months

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FKBP12 also known as FKBP-12 or FKBP12 protein is a small protein with a molecular weight of approximately 12 kDa. It belongs to the family of immunophilins and acts as a peptidyl-prolyl cis-trans isomerase (PPIase) facilitating the folding of proteins by catalyzing the isomerization of proline residues in polypeptides. The FKBP12 protein is expressed in various tissues throughout the body including the brain heart and skeletal muscles. It plays a mechanical role in binding certain macrolide antibiotics such as FK506 (tacrolimus) influencing their biological effects by altering protein conformation.

Biological function summary

FKBP12 is significant in cellular mechanisms beyond protein folding. It interacts with and forms complexes with other proteins such as ryanodine receptors and transforming growth factor-beta (TGF-β) receptors impacting calcium signaling and cell growth. FKBP12's interaction with FK506 has been extensively studied because it forms a complex that inhibits calcineurin ultimately leading to immunosuppressive effects. This property of FKBP12 makes it important for modulating the immune response and has importance in transplant medicine.

Pathways

FKBP12 is actively involved in calcium signaling and TGF-β signaling pathways. FKBP12 influences these pathways by binding to ryanodine receptors affecting calcium release from intracellular stores and also modulating the function of TGF-β receptors impacting cellular growth and proliferation processes. It is associated with proteins such as calcineurin and Smad proteins in these pathways maintaining cellular homeostasis and regulating immune and inflammatory responses.

FKBP12 plays a role in immunological and proliferative diseases. It is implicated in autoimmune disorders due to its interaction with FK506 leading to the suppression of unwanted immune responses. FKBP12 is also linked to cardiac hypertrophy as its modulation of calcium release through ryanodine receptors can affect heart muscle function. The protein interacts with calcineurin in these contexts contributing to pathological processes in these diseases. Researchers target FKBP12 with ELISA and other assays to investigate its involvement in these disorders and assess therapeutic interventions.

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製品プロトコール

Visit the General protocols
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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 50:344-363 PubMed40297641

2025

Multifunctional piezoelectric hydrogels under ultrasound stimulation boost chondrogenesis by recruiting autologous stem cells and activating the Ca/CaM/CaN signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Bao Liu,Xu Liu,Xiao-Fei Li,Liang Qiao,Hao-Liang Wang,Yue-Fu Dong,Feng Zhang,Yang Liu,Hao-Yang Liu,Ming-Liang Ji,Lan Li,Qing Jiang,Jun Lu

iScience 28:111555 PubMed39811636

2025

OsFKBP12 transduces the sucrose signal from OsNIN8 to the OsTOR pathway in a loosely binding manner for cell division.

Applications

Unspecified application

Species

Unspecified reactive species

Zizhang Wang,Hao Li,Yuxiang Weng

Acta neuropathologica communications 12:161 PubMed39390590

2024

A pathogenic mutation in the ALS/FTD gene VCP induces mitochondrial hypermetabolism by modulating the permeability transition pore.

Applications

Unspecified application

Species

Unspecified reactive species

Silke Vanderhaeghe,Jovan Prerad,Arun Kumar Tharkeshwar,Elien Goethals,Katlijn Vints,Jimmy Beckers,Wendy Scheveneels,Eveline Debroux,Katrien Princen,Philip Van Damme,Marc Fivaz,Gerard Griffioen,Ludo Van Den Bosch

The European respiratory journal 57: PubMed33033147

2020

The mononuclear phagocyte system contributes to fibrosis in post-transplant obliterans bronchiolitis.

Applications

Unspecified application

Species

Unspecified reactive species

Maria-Pia Di Campli,Abdulkader Azouz,Assiya Assabban,Jessika Scaillet,Marion Splittgerber,Alexandra Van Keymeulen,Frederick Libert,Myriam Remmelink,Alain Le Moine,Philippe Lemaitre,Stanislas Goriely

Autophagy 16:1683-1696 PubMed31826695

2019

Oxidation of multiple MiT/TFE transcription factors links oxidative stress to transcriptional control of autophagy and lysosome biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongfeng Wang,Nana Wang,Delai Xu,Qilian Ma,Yang Chen,Shiqiang Xu,Qin Xia,Yan Zhang,Jochen H M Prehn,Guanghui Wang,Zheng Ying

Acta ophthalmologica 97:e688-e699 PubMed30593729

2018

Survival and functionality of xeno-free human embryonic stem cell-derived retinal pigment epithelial cells on polyester substrate after transplantation in rabbits.

Applications

Unspecified application

Species

Unspecified reactive species

Tanja Ilmarinen,Fabian Thieltges,Heidi Hongisto,Kati Juuti-Uusitalo,Arto Koistinen,Kai Kaarniranta,Ralf Brinken,Norbert Braun,Frank G Holz,Heli Skottman,Boris V Stanzel

Scientific reports 7:8888 PubMed28827782

2017

Extracellular calcium modulates brown adipocyte differentiation and identity.

Applications

Unspecified application

Species

Unspecified reactive species

Ines Pramme-Steinwachs,Martin Jastroch,Siegfried Ussar

Molecular medicine reports 15:3001-3010 PubMed28339015

2017

Protection of FK506 against neuronal apoptosis and axonal injury following experimental diffuse axonal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Qin Huang,Jin-Ning Song,Feng-Wei Zheng,Hong-Gang Pang,Yong-Lin Zhao,Hua Gu,Jun-Jie Zhao

Journal of immunology (Baltimore, Md. : 1950) 197:3504-3511 PubMed27694496

2016

Antibody Binding to CD4 Induces Rac GTPase Activation and Alters T Cell Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Y Maurice Morillon,Elizabeth Chase Lessey-Morillon,Matthew Clark,Rui Zhang,Bo Wang,Keith Burridge,Roland Tisch

The Journal of neuroscience : the official journal of the Society for Neuroscience 35:14756-70 PubMed26538647

2015

An Endocytic Scaffolding Protein together with Synapsin Regulates Synaptic Vesicle Clustering in the Drosophila Neuromuscular Junction.

Applications

Unspecified application

Species

Unspecified reactive species

Åsa M E Winther,Olga Vorontsova,Kathryn A Rees,Tuomas Näreoja,Elena Sopova,Wei Jiao,Oleg Shupliakov

View all publications

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FK506 (Tacrolimus), Immunosuppressant