FFN511, Fluorescent substrate for VMAT2 (ab120331)
Key features and details
- First novel fluorescent false neurotransmitter. Enables optical imaging of presynaptic terminal activity.
- CAS Number: 1004548-96-2
- Soluble in DMSO to 100 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
FFN511, Fluorescent substrate for VMAT2 -
製品の詳細
First novel fluorescent false neurotransmitter. Enables optical imaging of presynaptic terminal activity. -
別名
- Fluorescent False Neurotransmitter 511
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生理活性の詳細
First novel fluorescent false neurotransmitter. Enables optical imaging of presynaptic terminal activity.
Inhibits 5-HT binding to VMAT2 (IC50 = 1 µM (comparable to dopamine itself)). Released via exocytosis in the striatum in mouse acute slice preparation. Labels dopamine and other presynaptic terminals in the striatum.
Compatible with GFP tags and other optical probes. Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy.
View the technique online
For highly selective labelling of dopaminergic presynaptic termainals FFN102 (ab120866) is recommended. -
CAS 番号
1004548-96-2 -
構造式
製品の特性
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体系名
9-(2-Aminoethyl)-2,3,6,7-tetrahydro-1H,5H,11H-[1]benzopyrano[6,7,8-ij]quinolizin-11-one -
分子量
284.36 -
分子式
C17H20N2O2 -
PubChem 登録番号
23725121 -
保存方法
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM and in ethanol to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
To make a 10 μM solution for slice incubation, 25 μl of a 20 mM FFN511 stock solution in DMSO is added to 50 ml of ACSF. This results in a 10 μM FFN511 solution in ACSF containing 0.05% DMSO.Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
NCCC2=CC(=O)Oc1c3CCCN4CCCc(cc12)c34 -
由来
Synthetic
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研究分野
画像
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Chemical Structure - FFN511, Fluorescent substrate for VMAT2 (ab120331)2D chemical structure image of ab120331, FFN511, Fluorescent substrate for VMAT2
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (6)
ab120331 は 6 報の論文で使用されています。
- Morales A et al. PACAP and acetylcholine cause distinct Ca2+ signals and secretory responses in chromaffin cells. J Gen Physiol 155:N/A (2023). PubMed: 36538657
- Chen X et al. Phospholipase C-ε defines a PACAP-stimulated pathway for secretion in the chromaffin cell. J Neuroendocrinol 35:e13255 (2023). PubMed: 36970756
- Guo X et al. Real-time visualization of exo- and endocytosis membrane dynamics with confocal and super-resolution microscopy. STAR Protoc 3:101404 (2022). PubMed: 35600934
- Paul N et al. Activation of the glucocorticoid receptor rapidly triggers calcium-dependent serotonin release in vitro. CNS Neurosci Ther 27:753-764 (2021). PubMed: 33715314
- Huang M et al. Drug testing complementary metal-oxide-semiconductor chip reveals drug modulation of transmitter release for potential therapeutic applications. J Neurochem 151:38-49 (2019). PubMed: 31274190
- Shin W et al. Visualization of Membrane Pore in Live Cells Reveals a Dynamic-Pore Theory Governing Fusion and Endocytosis. Cell 173:934-945.e12 (2018). PubMed: 29606354
