FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)
Key features and details
- Novel pH responsive FFN. Measures localization and activity of dopaminergic presynaptic terminals.
- CAS Number: 1234064-11-9
- Purity: > 98%
- Soluble in water to 50 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate -
製品の詳細
Novel pH responsive FFN. Measures localization and activity of dopaminergic presynaptic terminals. -
生理活性の詳細
Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent DAT and VMAT2 substrate. Enables two-photon microscopic imaging of localization and activity of dopaminergic presynaptic terminals in the striatum of mouse acute brain slice. More selective for dopaminergic synapses than FFN511 (ab120331).
Exhibits greater fluorescence emission in neutral than acidic environments allowing optical measurement of synaptic vesicle content release.
Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags. -
精製度
> 98% -
CAS 番号
1234064-11-9 -
構造式
製品の特性
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体系名
4-(2-Aminoethyl)-6-chloro-7-hydroxy-2H-1-benzopyran-2-one 2,2,2-trifluoroacetate -
分子量
353.68 -
分子式
C11H10ClNO3.CF3CO2H -
PubChem 登録番号
91885430 -
保存方法
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 50 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
C1=C(C2=CC(=C(C=C2OC1=O)O)Cl)CCN.C(=O)(C(F)(F)F)O -
由来
Synthetic
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研究分野
画像
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Chemical Structure - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)2D chemical structure image of ab120866, FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate
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Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)All images provided by Thorsten Lau
Figure 1: Two neuronal cells stained with 50 μM FFN102 on differentiation day 10. Shown is a sum projection of a confocal z-stack. Accumulation of FFN102 can be observed along the neurites (arrows) and the cell soma (S).
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Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)Figure 2a: Images in the first row show a group of neuronal cells stained with 50 μM FFN102 (sum projection of a confocal stack). FFN102 localizes to structures on the cell soma (S) as well as neurites (arrows). Z indicates the area zoomed in for an additional z-stack.
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Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)Figure 2b: Zoomed in area of “Z” from figure 2b. The arrow indicates a globular structure on a neurite. S indicates FFN102 positive structures on the cell soma. -
Cellular activation - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)Figure 3: FFN102 dye uptake inhibition on addition of VMAT2 inhibitor RO4-1284
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (3)
ab120866 は 3 報の論文で使用されています。
- Virdi GS et al. Protein aggregation and calcium dysregulation are hallmarks of familial Parkinson's disease in midbrain dopaminergic neurons. NPJ Parkinsons Dis 8:162 (2022). PubMed: 36424392
- Ho T et al. Vesicular expression and release of ATP from dopaminergic neurons of the mouse retina and midbrain. Front Cell Neurosci 9:389 (2015). PubMed: 26500494
- Rodriguez PC et al. Fluorescent dopamine tracer resolves individual dopaminergic synapses and their activity in the brain. Proc Natl Acad Sci U S A 110:870-5 (2013). PubMed: 23277566
