FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)
Key features and details
- Potent mitochondrial oxidative phosphorylation uncoupler
- CAS Number: 370-86-5
- Purity: > 99%
Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
FCCP, mitochondrial oxidative phosphorylation uncoupler -
製品の詳細
Potent mitochondrial oxidative phosphorylation uncoupler -
生理活性の詳細
Potent mitochondrial oxidative phosphorylation uncoupler (IC50 = 20 nM). Disrupts ATP synthesis by transporting protons across mitochondrial inner membranes. Depolarises mitochondrial membrane potential.
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精製度
> 99% -
CAS 番号
370-86-5 -
構造式
製品の特性
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体系名
Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone
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分子量
254.17 -
PubChem 登録番号
3330 -
保存方法
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM
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使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
FC(F)(F)Oc1ccc(cc1)NN=C(/C#N)C#N -
由来
Synthetic
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研究分野
関連製品
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Alternative Versions
画像
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Chemical Structure - FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)2D chemical structure image of ab120081, FCCP, mitochondrial oxidative phosphorylation uncoupler
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Cellular activation - FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)Image from Chan G K Y, et al. Plos One, 8(5), e63583. Fig S4,; doi: 10.1371/journal.pone.0063583HT29 cells were treated with the indicated compounds ((etoposide, 10 µM; gemcitabine 0.1 µM; paclitaxel 0.01 µM; PD901 1 µM, VX-680 0.2 µM) for 24 hours before analysis of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) using the Seahorse XF96 extracellular flux analyzer. Baseline rates (black) were determined at the indicated times before the addition of oligomycin (green) and then FCCP (red). Rate data are normalized to per-well cell number determined by post-analysis high-content imaging.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (40)
ab120081 は 40 報の論文で使用されています。
- Da Silva A et al. N-acetylneuraminate pyruvate lyase controls sialylation of muscle glycoproteins essential for muscle regeneration and function. Sci Adv 9:eade6308 (2023). PubMed: 37390204
- Skates E et al. Thioflavin T indicates mitochondrial membrane potential in mammalian cells. Biophys Rep (N Y) 3:100134 (2023). PubMed: 38026684
- Kristiansen CK et al. Comparing the mitochondrial signatures in ESCs and iPSCs and their neural derivations. Cell Cycle 21:2206-2221 (2022). PubMed: 35815665
- Sautchuk R et al. Transcriptional regulation of cyclophilin D by BMP/Smad signaling and its role in osteogenic differentiation. Elife 11:N/A (2022). PubMed: 35635445
- Moya ELJ et al. Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells. Int J Pharm 621:121780 (2022). PubMed: 35504427