Elastase Substrate, Fluorogenic (MeOSuc-AAPV-AFC) (ab142178)
Key features and details
- Highly sensitive coumarin-fluorogenic substrate for neutrophil elastase
- Purity: > 99%
Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Elastase Substrate, Fluorogenic (MeOSuc-AAPV-AFC) -
製品の詳細
Highly sensitive coumarin-fluorogenic substrate for neutrophil elastase -
生理活性の詳細
Highly sensitive coumarin-fluorogenic substrate for neutrophil elastase (Km =130 µM for leukocyte elastase). Compatible with in vitro assays with purified enzyme, on live cells, or in conditioned medium. The excitation wavelength is 380 nm and the emission wavelength is 500 nm.
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精製度
> 99% -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 1975 Elastase Substrate, Fluorogenic. 1975-5 is the same size as the 5 mg size of ab142178.
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構造式
製品の特性
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体系名
N-(Methoxysuccinyl)-Ala-Ala-Pro-Val-7-amino-4-trifluormethylcoumarin
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分子量
681.67 -
分子式
C31H38F3N5O9 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO
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使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
O=C(OC)CCC(=O)NC(C)C(=O)NC(C)C(=O)N1CCCC1C(=O)NC(C(C)C)C(=O)Nc2ccc3c(c2)OC(=O)C=C3C(F)(F)F -
由来
Synthetic
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研究分野
画像
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Chemical Structure - Elastase Substrate, Fluorogenic (MeOSuc-AAPV-AFC) (ab142178)2D chemical structure image of ab142178, Elastase Substrate, Fluorogenic (MeOSuc-AAPV-AFC)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab142178 は 2 報の論文で使用されています。
- Xu Y et al. Synthesis and evaluation of benzenesulfonic acid derivatives as human neutrophil elastase (hNE) inhibitors. Med Chem Res N/A:1-12 (2021). PubMed: 33456292
- Tokuhiro T et al. Oxidized Phospholipids and Neutrophil Elastase Coordinately Play Critical Roles in NET Formation. Front Cell Dev Biol 9:718586 (2021). PubMed: 34568331
