Dynole® 34-2, dynamin I and dynamin II inhibitor

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Dynole® 34-2, dynamin I and dynamin II inhibitor (AB120463)

ab66705 staining PAI1 in HeLa cells treated with dynole-34-2™; (ab120463), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynole-34-2™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120463 (dynole-34-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

• FuncS

PubMed

Functional Studies - Dynole® 34-2, dynamin I and dynamin II inhibitor (AB120463)

Primary human monocyte-derived macrophages (HMDM) were incubated with 30 μM of ab120463 for indicated time periods to determine if Dynamin inhibition decreases apoE secretion from primary human macrophages. Dynole-34-2 both dose- and time-dependently decreased apoE secretion without affecting HMDM viability (botton line on the graph as appose to the control, top line).

Kockx et al., PLOS One., 9(10) : e111186. Fig 1F.; doi : https : //doi.org/10.1371/journal.pone.0111186.

Image from Kockx et al., PLOS One., 9(10): e111186. Fig 1F.; doi: https://doi.org/10.1371/journal.pone.0111186. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• Chemical Structure

Lab

Chemical Structure - Dynole® 34-2, dynamin I and dynamin II inhibitor (AB120463)

2D chemical structure image of ab120463, Dynole® 34-2, dynamin I and dynamin II inhibitor