Dyngo® 4a, Novel, highly potent dynamin inhibitor (ab120689)
Key features and details
- Novel, highly potent dynamin inhibitor. Dynasore (ab120192) analog.
- CAS Number: 1256493-34-1
Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Dyngo® 4a, Novel, highly potent dynamin inhibitor -
製品の詳細
Novel, highly potent dynamin inhibitor. Dynasore (ab120192) analog. -
生理活性の詳細
Novel, highly potent dynamin inhibitor (IC50 values are 400 nM and 200 nM at dynamin I and II human-recombinant, respectively). Inhibits clathrin-mediated endocytosis (IC50 = 5.5 µM). Dynasore (ab120192) analog. Prevents botulin neurotoxin A (BoNTs) uptake and internalization, and prevents SNAP25 cleavage. Inhibits BoNTs induced paralysis and delays botulism onset.
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特記事項
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dyngo® is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.
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CAS 番号
1256493-34-1 -
構造式
製品の特性
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体系名
3-Hydroxy-N'-[(2,4,5-trihydroxyphenyl)methylidene]naphthalene-2-carbohydrazide
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分子量
338.31 -
分子式
C18H14N2O5 -
PubChem 登録番号
136227923 -
保存方法
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM
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使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
C1=CC=C2C=C(C(=CC2=C1)C(=O)N/N=C/C3=CC(=C(C=C3O)O)O)O -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120689の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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Chemical Structure - Dyngo® 4a, Novel, highly potent dynamin inhibitor (ab120689)2D chemical structure image of ab120689, Dyngo® 4a, Novel, highly potentdynamin inhibitor
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Functional Studies - Dyngo® 4a, Novel, highly potent dynamin inhibitor (ab120689)Kockx M et al. PLoS One. 2014; 9(10): e111186. doi: 10.1371/journal.pone.0111186 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Primary human monocyte-derived macrophages were treated without (Ctrl) and with ab120689 (Dyngo 4A) for 2 h. Effects on α-tubulin and acetylated tubulin (Ac-tubulin) were determined by confocal microscopy.
Credit: Kockx M et al. PLoS One. 2014; 9(10): e111186. doi: 10.1371/journal.pone.0111186
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Immunocytochemistry/ Immunofluorescence - Dyngo® 4a, Novel, highly potent dynamin inhibitor (ab120689)ab66705 staining PAI1 in HeLa cells treated with dyngo-4a™(ab120689), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dyngo-4a™, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120689 (dyngo-4a™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (78)
ab120689 は 78 報の論文で使用されています。
- Pan J et al. Differential Efficacy of Small Molecules Dynasore and Mdivi-1 for the Treatment of Dry Eye Epitheliopathy or as a Countermeasure for Nitrogen Mustard Exposure of the Ocular Surface. J Pharmacol Exp Ther 388:506-517 (2024). PubMed: 37442618
- Resnik N et al. Selective targeting of lectins and their macropinocytosis in urothelial tumours: translation from in vitro to ex vivo. Histochem Cell Biol 160:435-452 (2023). PubMed: 37535087
- Blythe EE & von Zastrow M β-Arrestin-independent endosomal cAMP signaling by a polypeptide hormone GPCR. Nat Chem Biol N/A:N/A (2023). PubMed: 37749347
- Grassi D et al. Nanoscale and functional heterogeneity of the hippocampal extracellular space. Cell Rep 42:112478 (2023). PubMed: 37149864
- Pizzoni A et al. Soluble cyclase-mediated nuclear cAMP synthesis is sufficient for cell proliferation. Proc Natl Acad Sci U S A 120:e2208749120 (2023). PubMed: 36656863