Dynasore, dynamin inhibitor (ab120192)
Key features and details
- Cell-permeable dynamin inhibitor
- CAS Number: 304448-55-3
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Dynasore, dynamin inhibitor -
製品の詳細
Cell-permeable dynamin inhibitor -
生理活性の詳細
Cell-permeable dynamin inhibitor, that blocks endocytosis. Non-competitively inhibits dynamin1, dynamin2 and mitochondrial dynamin GTPase activity. Selective over other small GTPases.
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CAS 番号
304448-55-3 -
構造式
製品の特性
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体系名
3-Hydroxynaphthalene-2-carboxylic acid (3,4-dihydroxybenzylidene)hydrazide -
分子量
322.31 -
分子式
C18H14N2O4 -
PubChem 登録番号
135533054 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
Oc3ccc(/C=N/NC(=O)c1cc2ccccc2cc1O)cc3O -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120192の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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Chemical Structure - Dynasore, dynamin inhibitor (ab120192)2D chemical structure image of ab120192, Dynasore, dynamin inhibitor
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Immunocytochemistry/ Immunofluorescence - Dynasore, dynamin inhibitor (ab120192)ab66705 staining PAI1 in HeLa cells treated with dynasore (ab120192), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynasore, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120689 (dynasore) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Western blot - Dynasore, dynamin inhibitor (ab120192)Jens H et al. PLoS One. 2016; 11(10): e0164768. doi: 10.1371/journal.pone.0164768 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/Western blots of indicated proteins after knockdown with gene-specific siRNA against AP2M1 (ab120192) or non-targeting siRNA transfection control (NT).
siRNAs were transfected into HMVEC-L at the concentration of 100 nM for 48h. The cells were then infected with ANDV (MOI = 0.5) for 24 h or 48 h. Western blots were performed post infection to ensure knockdown of the specific protein expression. The blots were also probed with β-actin specific antibody as the gel-loading control.
Jens H et al. PLoS One. 2016; 11(10): e0164768. doi: 10.1371/journal.pone.0164768
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (54)
ab120192 は 54 報の論文で使用されています。
- Zhang C et al. Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish. Nature 625:126-133 (2024). PubMed: 38123680
- Becker M et al. Efficient clathrin-mediated entry of enteric adenoviruses in human duodenal cells. J Virol 97:e0077023 (2023). PubMed: 37823645
- Mathiesen A et al. Adipose Tissue-Derived Extracellular Vesicles Contribute to Phenotypic Plasticity of Prostate Cancer Cells. Int J Mol Sci 24:N/A (2023). PubMed: 36674745
- Pantazopoulou M et al. Differential intracellular trafficking of extracellular vesicles in microglia and astrocytes. Cell Mol Life Sci 80:193 (2023). PubMed: 37391572
- Chen XN et al. Porcine Enteric Alphacoronavirus Entry through Multiple Pathways (Caveolae, Clathrin, and Macropinocytosis) Requires Rab GTPases for Endosomal Transport. J Virol 97:e0021023 (2023). PubMed: 36975780
