Dienogest, Steroidal progesterone (ab141282)
Key features and details
- Steroidal progesterone
- CAS Number: 65928-58-7
- Purity: > 99%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
-
製品名
Dienogest, Steroidal progesterone -
製品の詳細
Steroidal progesterone -
生理活性の詳細
Steroidal progesterone. Non-ethinylated progestin structurally related to testosterone. Anti-androgenic and antiproliferative effects in vitro.
-
精製度
> 99% -
CAS 番号
65928-58-7 -
構造式
製品の特性
-
体系名
(17α)-17-Hydroxy-3-oxo-19-norpregna-4,9-diene-21-nitrile -
分子量
311.42 -
分子式
C20H25NO2 -
PubChem 登録番号
68861 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES 線形表記
O=C3C=C4CC[C@@H]2C(CC[C@@]1(C)[C@H]2CC[C@@]1(O)CC#N)=C4CC3 -
由来
Synthetic
-
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab141282の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
画像
-
Chemical Structure - Dienogest, Steroidal progesterone (ab141282)2D chemical structure image of ab141282, Dienogest, Steroidal progesterone
-
Functional Studies - Dienogest, Steroidal progesterone (ab141282)ab16895 staining MDM2 in MCF7 cells treated with dienogest (ab141282), by ICC/IF. Decrease in MDM2 expression correlates with increased concentration of dienogest, as described in literature.
The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab141282 (dienogest) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab16895 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (1)
ab141282 は 1 報の論文で使用されています。
- Choi J et al. Dienogest enhances autophagy induction in endometriotic cells by impairing activation of AKT, ERK1/2, and mTOR. Fertil Steril 104:655-64.e1 (2015). PubMed: 26051103