Cryptotanshinone, STAT3 inhibitor (ab120666)
Key features and details
- STAT3 inhibitor with diverse biological activities
- CAS Number: 35825-57-1
- Purity: > 95%
- Soluble in DMSO to 10 mM
- Form / State: Solid
- Source: Salvia miltiorrhiza
製品の概要
-
製品名
Cryptotanshinone, STAT3 inhibitor -
製品の詳細
STAT3 inhibitor with diverse biological activities -
生理活性の詳細
STAT3 inhibitor (IC50 = 4.6 μM). Cell-permeable, naturally occuring constitutent of Salvia miltiorrhiza with diverse biological activities. Shows anticancer, antibacterial, anti-inflammatory, antidiabetes and antiobesity activity. Attenuates amyloid plaque deposition in the brain.
-
精製度
> 95% -
CAS 番号
35825-57-1 -
構造式
製品の特性
-
体系名
(R)-(-)-1,6,6-Trimethyl-1,2,6,7,8,9-hexahydrophenanthro[1,2-b]furan-10,11-dione -
分子量
296.37 -
分子式
C19H20O3 -
保存方法
Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions. -
溶解性
Soluble in DMSO to 10 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
由来
Salvia miltiorrhiza
-
研究分野
- Biochemicals
- Research Area
- Pain & inflammation
- Signaling
- Cytokine and NF-κB signaling
- STAT
- Inhibitors
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- JAK/STAT signaling
- STAT
- Inhibitors
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- JAK/STAT signaling
- STAT
- Inhibitors
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- JAK/STAT signaling
- STAT
- Inhibitors
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120666の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
画像
-
Chemical Structure - Cryptotanshinone, STAT3 inhibitor (ab120666)2D chemical structure image of ab120666, Cryptotanshinone, STAT3 inhibitor
-
Functional Studies - Cryptotanshinone, STAT3 inhibitor (ab120666)MCF7 cells were incubated at 37°C for 4h with vehicle control (0 µM) and different concentrations of cryptotanshinone (ab120666). Increased expression of JNK1+JNK2 (phospho T183 + Y185) in MCF7cells correlates with an increase in cryptotanshinone concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab4821 at at 1/1000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (1)
ab120666 は 1 報の論文で使用されています。
- Wang C et al. Cryptotanshinone Attenuates Airway Remodeling by Inhibiting Crosstalk Between Tumor Necrosis Factor-Like Weak Inducer of Apoptosis and Transforming Growth Factor Beta 1 Signaling Pathways in Asthma. Front Pharmacol 10:1338 (2019). PubMed: 31780948