Compound E, gamma;-secretase inhibitor (ab142164)
Key features and details
- Cell-permeable, selective, non-competitive, potent γ-secretase inhibitor
- CAS Number: 209986-17-4
- Purity: > 99%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Compound E, gamma;-secretase inhibitor -
製品の詳細
Cell-permeable, selective, non-competitive, potent γ-secretase inhibitor -
生理活性の詳細
Cell-permeable, selective, non-competitive, potent γ-secretase inhibitor (IC50 = 0.3 nM). Active in vitro and in vivo.
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精製度
> 99% -
CAS 番号
209986-17-4 -
構造式
製品の特性
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体系名
N-[(1S)-2-[[(3S)-2,3-Dihydro-1-methyl-2-oxo-5-phenyl-1H-1,4-benzodiazepin-3-yl]amino]-1-methyl-2-oxoethyl]-3,5-difluorobenzeneacetamide -
分子量
490.51 -
分子式
C27H24F2N4O3 -
PubChem 登録番号
11306390 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
C[C@@H](C(=O)N[C@@H]1C(=O)N(C2=CC=CC=C2C(=N1)C3=CC=CC=C3)C)NC(=O)CC4=CC(=CC(=C4)F)F -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab142164の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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Chemical Structure - Compound E, gamma;-secretase inhibitor (ab142164)2D chemical structure image of ab142164, Compound E, gamma;-secretase inhibitor
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Functional Studies - Compound E, gamma;-secretase inhibitor (ab142164)This image is courtesy of an Abreview submitted by a verified customerKc167 cells were treated with varying concentrations of compound E (ab142164) in DMSO for 16hr at 25°C; DMSO only was used as the negative control. The cells were further incubated for 30 minutes with 4mM EGTA in PBS (in the presence of compound E), and were then lysed for analysis. To measure Notch activity, Notch targets E(spl)mβ-HLH and E(spl)m3-HLH mRNA levels were assayed. Data shows the fold change of mRNA levels of E(spl)mβ-HLH and E(spl)m3-HLH under different conditions, normalised to DMSO treatment (negative control). Notch activation by EGTA is abrogated by treatment with compound E.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (8)
ab142164 は 8 報の論文で使用されています。
- Weatherbee BAT et al. Distinct pathways drive anterior hypoblast specification in the implanting human embryo. Nat Cell Biol 26:353-365 (2024). PubMed: 38443567
- Alhashem Z et al. Notch controls the cell cycle to define leader versus follower identities during collective cell migration. Elife 11:N/A (2022). PubMed: 35438077
- Zhang YF et al. MicroRNA-582-5p Contributes to the Maintenance of Neural Stem Cells Through Inhibiting Secretory Protein FAM19A1. Front Cell Neurosci 16:866020 (2022). PubMed: 35685988
- Taga A et al. Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons. J Vis Exp N/A:N/A (2021). PubMed: 34515684
- Naylor RW et al. A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion. Elife 7:N/A (2018). PubMed: 30394875
- Kang Y et al. Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys. Cell Rep 25:2563-2576.e9 (2018). PubMed: 30485820
- Liu X et al. BMP9 overexpressing adipose-derived mesenchymal stem cells promote cartilage repair in osteoarthritis-affected knee joint via the Notch1/Jagged1 signaling pathway. Exp Ther Med 16:4623-4631 (2018). PubMed: 30542413
- Slaninova V et al. Notch stimulates growth by direct regulation of genes involved in the control of glycolysis and the tricarboxylic acid cycle. Open Biol 6:150155 (2016). PubMed: 26887408
