CGP 57380, MNK inhibitor (ab120365)
Key features and details
- MNK inhibitor
- CAS Number: 522629-08-9
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
CGP 57380, MNK inhibitor -
製品の詳細
MNK inhibitor -
生理活性の詳細
Low-molecular-weight kinase inhibitor of MAP-kinase interacting kinase-1 (MNK1) and MNK2 (IC50 values are 0.87 and 1.6 μM, respectively). Inhibits a range of other kinases (IC50 values in μM shown in brackets) including: CKI (0.51) Aurora B (2.5) DYRK3 (3.2) SGK1( 2.7) BRSK2 (1.1) and Lck (2.5).
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CAS 番号
522629-08-9 -
構造式
製品の特性
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体系名
N3-(4-Fluorophenyl)-1H-pyrazolo-[3,4-d]pyrimidine-3,4-diamine -
分子量
244.23 -
分子式
C11H9FN6 -
PubChem 登録番号
11644425 -
保存方法
Store at +4°C. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
Fc1ccc(cc1)Nc3nnc2ncnc(N)c23 -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120365の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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Chemical Structure - CGP 57380, MNK inhibitor (ab120365)2D chemical structure image of ab120365, CGP 57380, MNK inhibitor
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Immunocytochemistry/ Immunofluorescence - CGP 57380, MNK inhibitor (ab120365)ab76256 staining eIF4E (phospho S209) in serum starved HEK193 cells treated with CGP 57380 (ab120365), by ICC/IF. Decrease in eIF4E (phospho S209) expression correlates with increased concentration of CGP 57380, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120365 (CGP 57380) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76256 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (2)
ab120365 は 2 報の論文で使用されています。
- Moore CE et al. MNK1 and MNK2 mediate adverse effects of high-fat feeding in distinct ways. Sci Rep 6:23476 (2016). PubMed: 27087055
- Leong SY et al. The role of Misshapen NCK-related kinase (MINK), a novel Ste20 family kinase, in the IRES-mediated protein translation of human enterovirus 71. PLoS Pathog 11:e1004686 (2015). PubMed: 25747578