BrdU (5-bromo-2'-deoxyuridine), Thymidine analog

• Chemical Structure

Lab

Chemical Structure - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (AB142567)

2D chemical structure image of ab142567, BrdU (5-bromo-2'-deoxyuridine), Thymidine analog

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (AB142567)

ab220075 staining BrdU in HeLa cells. Untreated and BrdU treated (ab142567, 10uM for 24 hours) cells were fixed with 100% methanol (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab220075 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

• FuncS

PubMed

Functional Studies - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (AB142567)

Estimation of S-phase Duration using EdU-BrdU Dual Pulse Labelling.

(From Figure 5A of Massey)

Massey, AJ PLoS One. 2015 Jul 28;10(7):e0134306. doi: 10.1371/journal.pone.0134306. eCollection 2015. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/