AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)
Key features and details
- Cell-permeable activator of AMP-activated protein kinase
- CAS Number: 2627-69-2
- Soluble in water to 50 mM and in DMSO to 100 mM (with heating)
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
AICAR (Acadesine/AICA riboside), AMPK activator -
製品の詳細
Cell-permeable activator of AMP-activated protein kinase -
別名
- Acadesine
- AICA riboside
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生理活性の詳細
Cell-permeable activator of AMP-activated protein kinase. Is taken up into cells by adenosine transporters and phosphorylated by adenosine kinase to the active nucleotide ZMP (5-aminoimidazole-4-carboxamide ribonucleoside), which mimics effects of AMP on the AMPK system. Active in vivo and in vitro.
Also available in simple stock solutions (ab146713) - add 1 ml of water to get an exact, ready-to-use concentration. -
CAS 番号
2627-69-2 -
構造式
製品の特性
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体系名
5-Amino-1-[(2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl]-1H-imidazole-4-carboxamide -
分子量
258.23 -
分子式
C9H14N4O5 -
PubChem 登録番号
17513 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 50 mM and in DMSO to 100 mM (with heating) -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
NC(=O)c2ncn([C@@H]1O[C@H](CO)[C@H](O)[C@@H]1O)c2N -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120358の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
画像
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Chemical Structure - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)2D chemical structure image of ab120358, AICAR (Acadesine/AICA riboside), AMPK activator
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Functional Studies - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)ab39400 staining AMPK alpha 1 (phospho S496) in HepG2 cells treated with AICAR (ab120358), by ICC/IF. Increase in AMPK alpha 1 (phospho S496) expression correlates with increased concentration of AICAR, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120358 (AICAR) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab39400 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Western blot - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)Li F et al. PLoS One. 2015; 10(9): e0138390. doi: 10.1371/journal.pone.0138390 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/T24 (transitional cell carcinoma) and UMUC-3 cells were pre-incubated with G9a activator AICAR (1mM, ab120358) for 4 h and then treated with BIX-01294 (1.5 μM) for 24h. The levels of p-AMPKα, p-mTOR and LC-3 I/II was examined.
Credit: Li F et al. PLoS One. 2015; 10(9): e0138390. doi: 10.1371/journal.pone.0138390
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (17)
ab120358 は 17 報の論文で使用されています。
- Kakadia JH et al. AMPK-mTORC1 pathway mediates hepatic IGFBP-1 phosphorylation in glucose deprivation: a potential molecular mechanism of hypoglycemia-induced impaired fetal growth. J Mol Endocrinol 72:N/A (2024). PubMed: 38194365
- Vilchinskaya NA et al. AMPK Phosphorylation Impacts Apoptosis in Differentiating Myoblasts Isolated from Atrophied Rat Soleus Muscle. Cells 12:N/A (2023). PubMed: 36980261
- Sternlieb T et al. An AMP-activated protein kinase complex with two distinctive alpha subunits is involved in nutritional stress responses in Trypanosoma cruzi. PLoS Negl Trop Dis 15:e0009435 (2021). PubMed: 34029334
- Gao P et al. Inhibition of Mitochondrial Calcium Overload by SIRT3 Prevents Obesity- or Age-Related Whitening of Brown Adipose Tissue. Diabetes 69:165-180 (2020). PubMed: 31712319
- Gong Y et al. Metformin Inhibits Tumor Metastasis through Suppressing Hsp90a Secretion in an AMPKa1-PKC? Dependent Manner. Cells 9:N/A (2020). PubMed: 31936169