Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit (ab108915)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue
- Sensitivity: 0.0051 U/ml
製品の概要
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製品名
Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit
Urokinase キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Tissue, Cell Lysate -
アッセイタイプ
Enzyme activity (quantitative) -
検出感度
0.0051 U/ml -
種交差性
交差種: Human -
製品の概要
Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.
ab108915 uPA Human Chromogenic Activity Assay Kit is developed to determine Human uPA activity in plasma and cell culture supernatants. The assay measures the ability of uPA to activate the plasminogen to plasmin in coupled or indirect assays that contain uPA, plasminogen, and a plasmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA in the reaction solution at 405 nm is directly proportional to the uPA enzymatic activity.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Assay Diluent 1 x 30ml Human Plasminogen 1 x 2 vials Human uPA Standard 1 vial Microplate 1 unit Plasmin Substrate 1 x 2 vials Sealing Tapes 3 units -
研究分野
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機能
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
組織特異性
Expressed in the prostate gland and prostate cancers. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
翻訳後修飾
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
細胞内局在
Secreted. - Information by UniProt
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別名
- ATF
- ATF uPA
- BDPLT5
see all -
参照データベース
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- Unigene: 77274 Human
画像
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Indirect ELISA - Urokinase type plasminogen activator Human Chromogenic Activity Assay Kit (ab108915)
uPA measured in biologicals showing quantity (IU) per mL of sample tested.
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Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)uPA Chromogenic Activity Standard Curve (For High uPA Activity Samples)
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Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)uPA Chromogenic Activity Standard Curve (For Low uPA Activity Samples)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab108915 は 2 報の論文で使用されています。
- Hohensinner PJ et al. PAI-1 (Plasminogen Activator Inhibitor-1) Expression Renders Alternatively Activated Human Macrophages Proteolytically Quiescent. Arterioscler Thromb Vasc Biol N/A:N/A (2017). PubMed: 28818858
- Gupta V et al. Glaucoma is associated with plasmin proteolytic activation mediated through oxidative inactivation of neuroserpin. Sci Rep 7:8412 (2017). PubMed: 28827627