Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Nuclear Extracts, Purified protein, Serum
- Sensitivity: 0.02 IU/ml
製品名Urokinase Activity Assay Kit (Fluorometric)
Urokinase キット 製品一覧
サンプルの種類Serum, Cell culture extracts, Nuclear Extracts, Purified protein
アッセイタイプEnzyme activity (quantitative)
検出感度>= 0.02 IU/ml
種交差性交差種: Mammals, Other species
Urokinase Activity Assay Kit (Fluorometric) (ab174098) uses an AMC-based peptide substrate containing the recognition sequence for Urokinase. Urokinase present in the sample catalyzes the cleavage of the substrate and releases AMC, which can be easily quantified by measuring its fluorescence at Ex/Em = 350/450 nm. This sensitive assay detects very low levels of Urokinase activity in biological samples as well as purified enzymes (as low as 0.02 IU/ml).
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This product is manufactured by BioVision, an Abcam company and was previously called K728 Urokinase Activity Fluorometric Assay Kit. K728-100 is the same size as the 100 test size of ab174098.
Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.
保存方法Store at -20°C. Please refer to protocols.
内容 100 tests Cell Lysis Buffer 1 x 25ml Human Urokinase (Standard/Positive Control) 1 vial Urokinase Assay Buffer 1 x 25ml Urokinase Substrate 1 x 0.2ml
機能Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
組織特異性Expressed in the prostate gland and prostate cancers.
配列類似性Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.
翻訳後修飾Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
- Information by UniProt
- ATF uPA
ab174098 は 2 報の論文で使用されています。
- Hsieh HY et al. Hyaluronic acid on the urokinase sustained release with a hydrogel system composed of poloxamer 407: HA/P407 hydrogel system for drug delivery. PLoS One 15:e0227784 (2020). PubMed: 32160196
- Álvarez E et al. Protective, repairing and fibrinolytic effects of rivaroxaban on vascular endothelium. Br J Clin Pharmacol 84:280-291 (2018). PubMed: 28940408