Urokinase Activity Assay Kit (Fluorometric) (ab174098)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Nuclear Extracts, Purified protein, Serum
- Sensitivity: 0.02 IU/ml
製品の概要
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製品名
Urokinase Activity Assay Kit (Fluorometric)
Urokinase キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Serum, Cell culture extracts, Nuclear Extracts, Purified protein -
アッセイタイプ
Enzyme activity (quantitative) -
検出感度
>= 0.02 IU/ml -
種交差性
交差種: Mammals, Other species -
製品の概要
Urokinase Activity Assay Kit (Fluorometric) (ab174098) uses an AMC-based peptide substrate containing the recognition sequence for Urokinase. Urokinase present in the sample catalyzes the cleavage of the substrate and releases AMC, which can be easily quantified by measuring its fluorescence at Ex/Em = 350/450 nm. This sensitive assay detects very low levels of Urokinase activity in biological samples as well as purified enzymes (as low as 0.02 IU/ml).
Visit our FAQs page for tips and troubleshooting.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K728 Urokinase Activity Fluorometric Assay Kit. K728-100 is the same size as the 100 test size of ab174098.
Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Cell Lysis Buffer 1 x 25ml Human Urokinase (Standard/Positive Control) 1 vial Urokinase Assay Buffer 1 x 25ml Urokinase Substrate 1 x 0.2ml -
研究分野
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機能
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
組織特異性
Expressed in the prostate gland and prostate cancers. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
翻訳後修飾
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
細胞内局在
Secreted. - Information by UniProt
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別名
- ATF
- ATF uPA
- BDPLT5
see all
画像
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Urokinase Activity Standard Curves
This is example data only.
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Urokinase Activity Standard CurvesThis is example data only.
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Urokinase activities in various samplesUrokinase activities of Jurkat cell extract [10 µl], Hela nuclear extract [10 µl] and Pooled human serum (10 µl). Assays were performed following the kit protocol. This is example data only.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab174098 は 2 報の論文で使用されています。
- Hsieh HY et al. Hyaluronic acid on the urokinase sustained release with a hydrogel system composed of poloxamer 407: HA/P407 hydrogel system for drug delivery. PLoS One 15:e0227784 (2020). PubMed: 32160196
- Álvarez E et al. Protective, repairing and fibrinolytic effects of rivaroxaban on vascular endothelium. Br J Clin Pharmacol 84:280-291 (2018). PubMed: 28940408