Trichrome Stain Kit (Connective Tissue Stain) (ab150686)
製品の概要
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製品名
Trichrome Stain Kit (Connective Tissue Stain) -
製品の概要
Trichrome Stain Kit (Connective Tissue Stain) ab150686 is intended for use in the histological visualization of collagenous connective tissue fibers in tissue sections.
The trichrome stain kit may be used on formalin-fixed, paraffin-embedded. For frozen sections, it will require optimization by the user.
Trichrome stain protocol summary
- Deparaffinize sections if necessary
- Preheat Bouin's Fluid to 54-64°C
- Incubate slide in preheated Bouin's Fluid for 60 min and cool for 10 min
- Rinse in water
- Incubate slide in Weigert's Iron Hematoxylin for 5 min
- Rinse in water
- Incubate slide in Biebrich Scarlet / Aci0d Fuchsin solution for 15 min
- Rinse in water
- Differentiate in phosphomolybdic / phosphotungstic acid solution for 10-15 min
- Incubate slide in Aniline Blue solution for 5-10 min
- Rinse in water
- Incubate slide in acetic acid solution for 3-5 min
- Dehydrate, clear and mount
Staining Interpretation:
Collagen
Blue
Muscle Fibers
Red
Nuclei
Dark Red to Black/Blue
Control Tissue: Lung, Uterus, Small Intestine, Stomach.
Related and recommended products
See below other connective tissue stains to highlight and identify different types of connective tissue in tissue samples with different dyes:
-Picro Sirius Red Stain Kit (Connective Tissue Stain) ab150681
-Phosphotungstic Acid Hematoxylin (PTAH) Stain Kit (Connective Tissue Stain) ab150683
-Reticulum Stain Kit (Connective Tissue Stain) ab150684
製品の特性
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保存方法
Store at room temperature. Please refer to protocols. -
内容 100 tests Acetic Acid Solution (1%) 1 x 125ml Aniline Blue Solution 1 x 125ml Biebrich Scarlet/Acid Fuchsin Solution 1 x 125ml Bouin's Fluid 1 x 125ml Phosphomolybdic/Phosphotungstic acid solution 1 x 125ml Weigert's Iron, Hematoxylin (A) 1 x 125ml Weigert's Iron, Hematoxylin (B) 1 x 125ml -
研究分野
画像
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ab150686 - Trichrome Stain Kit (Connective Tissue Stain)
ab150686 Trichrome Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human muscle.
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ab150686 - Trichrome Stain Kit (Connective Tissue Stain)ab150686 (Trichrome Stain) staining collagen (blue) and muscle fibre (red) in formalin fixed paraffin embedded normal human placenta. Nuclei are counterstained with Weigert’s hematoxylin (blue)
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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ab150686 - Trichrome Stain Kit (Connective Tissue Stain)This image is courtesy of an Abreview submitted by Javier RodriguezA paraffin-embedded section of healthy mouse spleen stained with ab150686 - Trichrome Stain Kit (Connective Tissue Stain). The sample was deparaffinized by heating to 60°C for 1 hour and then deparaffinization following a standard protocol. Sections were then fixed with Bouin's solution (included in the kit) for 1 hour at 60°C in humid conditions.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (205)
ab150686 は 205 報の論文で使用されています。
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- Xiao F et al. Shenhuang plaster enhances intestinal anastomotic healing in rabbits through activation of the TGF-β and Hippo/YAP signaling pathways. J Appl Biomed 21:208-217 (2023). PubMed: 38112460
- Simonson B et al. Single-nucleus RNA sequencing in ischemic cardiomyopathy reveals common transcriptional profile underlying end-stage heart failure. Cell Rep 42:112086 (2023). PubMed: 36790929