AB150686

Trichrome Stain Kit (Connective Tissue Stain)

Name: Trichrome Stain Kit (Connective Tissue Stain) (ab150686) | Abcam
Brand: Abcam Limited
SKU: AB150686
Price: 72000 JPY
Availability: InStock
Rating: 5 (5 reviews)

(5 Reviews)

(283 Publications)

Trichrome Stain Kit ab150686 is a Masson's Trichrome Stain Kit for use in the histological visualization of collagenous connective tissue fibers in tissue sections.

- Ready to use solutions- Cited in >200 publications
- Includes complete reagents for Aniline Blue, Biebrich Scarlet/Acid Fuchsin, and Weigert's Iron Hematoxylin trichrome staining

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Trichrome Stain Kit (Connective Tissue Stain) (AB150686)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Trichrome Stain Kit (Connective Tissue Stain) (AB150686)

A paraffin-embedded section of healthy mouse spleen stained with ab150686 - Trichrome Stain Kit (Connective Tissue Stain). The sample was deparaffinized by heating to 60°C for 1 hour and then deparaffinization following a standard protocol. Sections were then fixed with Bouin's solution (included in the kit) for 1 hour at 60°C in humid conditions.

This image is courtesy of an Abreview submitted by Javier Rodriguez

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Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Trichrome Stain Kit (Connective Tissue Stain) (AB150686)

ab150686 Trichrome Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human muscle.

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Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Trichrome Stain Kit (Connective Tissue Stain) (AB150686)

ab150686 (Trichrome Stain) staining collagen (blue) and muscle fibre (red) in formalin fixed paraffin embedded normal human placenta. Nuclei are counterstained with Weigert's hematoxylin (blue)

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Key facts

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製品の詳細

Masson's Trichrome Stain Kit ab150686 for histological staining of collagen, collagen fibers, fibrin, muscles, and erythrocytes. Three stains are used in Masson's trichrome staining: Aniline blue, Biebrich scarlet-acid fuschin, and Weigert’s Hematoxylin. Aniline blue is provided in this kit; in some other protocols a green dye is used to stain collagen instead of Aniline blue.

The trichrome staining protocol used in this kit is designed for use on formalin-fixed, paraffin-embedded tissue sections. For frozen sections, it will require optimization by the user.

Masson's Trichrome staining protocol summary

Deparaffinize sections if necessary
Preheat Bouin's Fluid to 54-64°C
Incubate slide in preheated Bouin's Fluid for 60 min and cool for 10 min
Rinse in water
Incubate slide in Weigert's Iron Hematoxylin for 5 min
Rinse in water
Incubate slide in Biebrich Scarlet / Acid Fuchsin solution for 15 min
Rinse in water
Differentiate in phosphomolybdic / phosphotungstic acid solution for 10-15 min
Incubate slide in Aniline Blue solution for 5-10 min
Rinse in water
Incubate slide in acetic acid solution for 3-5 min
Dehydrate, clear and mount

Staining Interpretation

Collagen	Blue (Aniline Blue)
Muscle Fibers	Red (Biebrich scarlet-acid fuschin)
Nuclei	Dark Red to Black/Blue (Weigert’s Hematoxylin)

Control Tissue: Lung, Uterus, Small Intestine, Stomach.

Related and recommended products
See below other connective tissue stains to highlight and identify different types of connective tissue in tissue samples with different dyes:

Picro Sirius Red Stain Kit (Connective Tissue Stain) ab150681
Phosphotungstic Acid Hematoxylin (PTAH) Stain Kit (Connective Tissue Stain) ab150683
Reticulum Stain Kit (Connective Tissue Stain) ab150684

How other researchers use Masson's Trichrome Staining

Based on a brief survey of publications using Trichrome Stain Kit ab150686, Masson's Trichrome staining is most often used to stain for fibrosis and collagen levels in liver, heart, and lung. It is also often used with pancreas, bladder, kidney, skin, adipose tissue, and vascular tissue.

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製品内容

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出荷温度及び保存条件

出荷温度

Ambient - Can Ship with Ice

短期保存温度

Ambient

長期保存温度

Ambient

保管に関する情報

Ambient

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Collagenous connective tissue fibers often referred to simply as collagen fibers are essential structural components in various tissues including skin bone tendons and ligaments. These fibers with a molecular mass that can vary depending on the type of collagen provide tensile strength and support to tissues and organs. They are synthesized by fibroblasts and expressed abundantly in extracellular matrix environments. Collagenous fibers are identifiable using Masson's trichrome staining which helps differentiate these fibers in histological studies.

Biological function summary

Collagenous fibers contribute to the mechanical framework of connective tissues enabling them to resist stretching and deformation. They are part of larger complexes that include elastin and proteoglycans working together to maintain tissue integrity and function. These fibers interact with other cellular components through specific receptors like integrins which mediate signals for cellular processes including differentiation adhesion and migration.

Pathways

Collagenous connective tissue fibers play roles in wound healing and tissue remodeling. They participate in pathways involving matrix metalloproteinases (MMPs) which regulate the degradation of extracellular matrix components. The collagen synthesis and cross-linking process often links with proteins such as fibronectin and laminin which influence cell behavior and tissue repair mechanisms.

Defective or insufficient collagenous connective tissue fibers can lead to conditions like Ehlers-Danlos syndrome and osteogenesis imperfecta. Both disorders reflect abnormalities in collagen synthesis or structure resulting in increased tissue fragility and elasticity issues. Mutations in collagen-related genes often underlie these pathologies highlighting the importance of these fibers in maintaining structural and functional integrity of various tissues.

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製品プロトコール

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ターゲットの情報

See full target information Phosphomolybdicacid

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文献 (283)

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Trichrome Stain Kit (Connective Tissue Stain)