AB83393

Starch Assay Kit

Name: Starch Assay Kit (ab83393) | Abcam
Brand: Abcam Limited
SKU: AB83393
Rating: 4 (1 reviews)

(1 Review)

(5 Publications)

Abcam's Starch Assay Kit provides an easy, accurate assay to measure starch levels in a variety of samples.

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Functional Studies - Starch Assay Kit (AB83393)

FuncS

Supplier Data

Functional Studies - Starch Assay Kit (AB83393)

Starch Standard Curve : Different types of pure starch were extracted with 10N KOH/H₃PO₄ as described. Assays were performed following the kit protocol.

Key facts

検出方法

Colorimetric/Fluorometric

サンプルタイプ

Tissue Lysate, Food samples, Cell Lysate

アッセイタイプ

Quantitative

検出感度

> 4 µg/mL

検出範囲

4 - 2000 µg/mL

アッセイ時間

40m

アッセイプラットフォーム

Microplate reader

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製品の詳細

Abcam's Starch Assay Kit provides an easy, accurate assay to measure starch levels in a variety of samples. In the assay, starch is hydrolyzed to glucose which is oxidized to generate color (570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay can detect starch at 0.0004 to 2 mg/ml.

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K647 Starch Colorimetric/Fluorometric Assay Kit. K647-100 is the same size as the 100 test size of ab83393.

Starch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear α-1,4 linked polymer) and amylopectin, (highly α-1,6 branched α-1,4 polymer). Starch generally contains 0-25% amylose and 75-100% amylopectin.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Starch is a polysaccharide consisting of numerous glucose units joined together by glycosidic bonds. It is commonly found in plants and serves as a major form of energy storage. Starch comprises two components: amylose which is mostly linear and amylopectin which is highly branched. Its molecular mass varies depending on the ratios of amylose to amylopectin. Starch accumulates in various plant tissues particularly in seeds tubers and roots where it functions as a reserve of carbohydrates to be used when photosynthesis is not possible.

Biological function summary

Starch plays a significant role as an energy source in the dietary intake of humans and animals. It is part of the carbohydrate storage complex in plants. Animals and humans break down starch using specific enzymes such as amylase and maltase converting it into glucose for energy growth and cellular function. This stored energy becomes essential for processes such as germination in plants and demands of high metabolic activities in animals. Starch's role in nutrition is critical among both domestic and wild species.

Pathways

Starch is involved in key metabolic routes such as the glycolysis and gluconeogenesis. In animals and humans enzymes like alpha-amylase initiate its breakdown paving the way for glucose to enter glycolytic pathways for ATP production whereas plant metabolism involves enzymes such as starch synthase within chloroplasts. Glycolysis enables efficient energy release from the stored glucose derived from starch linking to insulin and other regulatory proteins thereby integrating with broader metabolic networks.

Starch digestion-related abnormalities such as congenital sucrase-isomaltase deficiency and type 2 diabetes have notable connections. In the case of diabetes improper starch metabolism leads to hyperglycemia due to inadequate insulin activity or production and involves proteins such as insulin and glucagon. These disorders highlight the importance of controlled starch breakdown and absorption processes in maintaining normal physiological functions highlighting the potential for starch-related interventions in disease management.

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製品プロトコール

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ターゲットの情報

See full target information Amylopectin

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35409249

2022

Exploring the Contribution of Autophagy to the Excess-Sucrose Response in .

Applications

Unspecified application

Species

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Daniel Laloum,Sahar Magen,Yoram Soroka,Tamar Avin-Wittenberg

Frontiers in plant science 8:2070 PubMed29312368

2017

Regulation of Carbon Partitioning in the Seed of the Model Legume and : A Comparative Approach.

Applications

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Species

Unspecified reactive species

Youhong Song,Liang He,Xin-Ding Wang,Nathan Smith,Simon Wheeler,Manohar L Garg,Ray J Rose

Plant & cell physiology 56:2297-311 PubMed26468507

2015

SIZ1-Dependent Post-Translational Modification by SUMO Modulates Sugar Signaling and Metabolism in Arabidopsis thaliana.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Humberto Castro,Nuno Verde,Tiago Lourenço,Alexandre Papadopoulos Magalhães,Rui Manuel Tavares,Eduardo Rodríguez Bejarano,Herlânder Azevedo

Journal of experimental botany 65:4217-39 PubMed24821950

2014

TOPOISOMERASE 6B is involved in chromatin remodelling associated with control of carbon partitioning into secondary metabolites and cell walls, and epidermal morphogenesis in Arabidopsis.

Applications

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Species

Unspecified reactive species

Amandeep Mittal,Rajagopal Balasubramanian,Jin Cao,Prabhjeet Singh,Senthil Subramanian,Glenn Hicks,Eugene A Nothnagel,Noureddine Abidi,Jaroslav Janda,David W Galbraith,Christopher D Rock

Tree physiology 33:1338-53 PubMed24336517

2013

Size-dependent changes in leaf and wood chemical traits in two Caribbean rainforest trees.

Applications

Unspecified application

Species

Unspecified reactive species

Adam R Martin,Sean C Thomas

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Starch Assay Kit

Starch Assay Kit

Functional Studies - Starch Assay Kit (AB83393)

Key facts

検出方法

サンプルタイプ

アッセイタイプ

検出感度

検出範囲

アッセイ時間

アッセイプラットフォーム

製品の詳細

製品内容

出荷温度及び保存条件

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

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