AB138877

Sphingomyelin Assay Kit (Fluorometric)

Name: Sphingomyelin Assay Kit (Fluorometric) (ab138877) | Abcam
Brand: Abcam Limited
SKU: AB138877
Price: 74000 JPY
Availability: InStock

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(6 Publications)

Abcam's Sphingomyelin Assay Kit (Fluorometric) (ab138877) provides the most sensitive method for detecting neutral SM activity or screening SM inhibitors.

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Functional Studies - Sphingomyelin Assay Kit (Fluorometric) (AB138877)

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Supplier Data

Functional Studies - Sphingomyelin Assay Kit (Fluorometric) (AB138877)

Sample Standard Curve for Sphingomyelin. Sphingomyelin dose response was measured on a solid black 96-well plate with ab138877 using a fluorescence microplate reader. As low as 1 µM sphingomyelin can be detected with 60 minutes incubation (n=3).

Key facts

検出方法

Fluorescent

サンプルタイプ

Cell culture extracts, Whole Blood

検出感度

= 1 µM

アッセイプラットフォーム

Microplate reader

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製品の詳細

Abcam's Sphingomyelin Assay Kit (Fluorometric) (ab138877) provides the most sensitive method for detecting neutral SM activity or screening SM inhibitors. The kit uses AbRed Indicator as a fluorogenic probe to indirectly quantify the phosphocholine produced from the hydrolysis of sphingomyelin (SM) by sphingomyelinase (SMase). It can be used for measuring the amount of SM in blood, cell extracts or other solutions. The fluorescence intensity of AbRed Indicator is proportional to the formation of phosphocholine, therefore to the amount of SM. AbRed Indicator enables the assay readable by either a fluorescence reader or an absorbance reader.

The kit is an optimized "mix and read" assay that can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue. It plays an important role in signal transduction. Sphingomyelin accumulates abnormally in Niemann-Pick disease and Abetalipoproteinemia.

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Sphingomyelin often known as SM is a type of sphingolipid found in animal cell membranes particularly in the myelin sheath of nerve cells. It consists of a phosphocholine head group a sphingoid base often sphingosine and a fatty acid. Sphingomyelin has a molecular weight of approximately 750 Da. It generally resides in the plasma membrane where it contributes to structural integrity and participates in cell signaling processes. Human tissues exhibiting substantial expression include the brain and nervous system indicating its key role in neural function.

Biological function summary

Sphingomyelin is part of the cellular structure acting as both a lipid raft component and a reservoir for secondary messengers. Its breakdown into ceramide by sphingomyelinases influences cellular events such as proliferation differentiation and apoptosis. Sphingomyelin does not operate independently; it forms complexes with other lipids and proteins such as cholesterol to modulate membrane fluidity and receptor function. These actions have palpable impacts on signal transduction and cellular stress responses.

Pathways

Sphingomyelin plays a critical role in the sphingolipid signaling and metabolism pathways. It is a precursor in the sphingomyelinase pathway where it breaks down into ceramide. Ceramide acts as an essential signaling molecule influencing diverse biological processes including inflammation and cell death. This pathway also links with proteins like acid sphingomyelinase and neutral sphingomyelinase highlighting interactions essential for cell regulation.

Sphingomyelin imbalance or accumulation is associated with Niemann-Pick disease a rare lysosomal storage disorder. It also connects to atherosclerosis where its dynamic with ceramide impacts lipid metabolism and plaque stability. Proteins like Niemann-Pick disease type C1 and Niemann-Pick disease type C2 are directly involved in these conditions exemplifying the role of sphingomyelin in pathological mechanisms. Understanding these relationships aids in exploring therapeutic targets for relevant disorders.

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製品プロトコール

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ターゲットの情報

See full target information Sphingomyelin(43:7)

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 17:4141-4158 PubMed33749503

2021

Platelet autophagic machinery involved in thrombosis through a novel linkage of AMPK-MTOR to sphingolipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Tzu-Yin Lee,Wan-Jung Lu,Chun A Changou,Yuan-Chin Hsiung,Nguyen T T Trang,Cheng-Yang Lee,Tzu-Hao Chang,Thanasekaran Jayakumar,Cheng-Ying Hsieh,Chih-Hao Yang,Chao-Chien Chang,Ray-Jade Chen,Joen-Rong Sheu,Kuan-Hung Lin

eLife 9: PubMed33164744

2020

Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.

Applications

Unspecified application

Species

Unspecified reactive species

Alex J B Kreutzberger,Volker Kiessling,Catherine A Doyle,Noah Schenk,Clint M Upchurch,Margaret Elmer-Dixon,Amanda E Ward,Julia Preobraschenski,Syed S Hussein,Weronika Tomaka,Patrick Seelheim,Iman Kattan,Megan Harris,Binyong Liang,Anne K Kenworthy,Bimal N Desai,Norbert Leitinger,Arun Anantharam,J David Castle,Lukas K Tamm

Cancers 11: PubMed31756922

2019

The Transfer of Sphingomyelinase Contributes to Drug Resistance in Multiple Myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvia Faict,Inge Oudaert,Ludovic D'Auria,Jonas Dehairs,Ken Maes,Philip Vlummens,Kim De Veirman,Elke De Bruyne,Karel Fostier,Isabelle Vande Broek,Rik Schots,Karin Vanderkerken,Johannes V Swinnen,Eline Menu

Cell communication and signaling : CCS 17:54 PubMed31133022

2019

Crystal structure of a cytocidal protein from lamprey and its mechanism of action in the selective killing of cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Pang,Meng Gou,Kai Yang,Jiali Lu,Yinglun Han,Hongming Teng,Changzhi Li,Haina Wang,Caigang Liu,Kejia Zhang,Yongliang Yang,Qingwei Li

Nature communications 10:1387 PubMed30918259

2019

Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Ju Im,Chan-Hyeong Lee,Pyong-Gon Moon,Gunassekaran Gowri Rangaswamy,Byungheon Lee,Jae Man Lee,Jae-Chul Lee,Jun-Goo Jee,Jong-Sup Bae,Taeg-Kyu Kwon,Keon-Wook Kang,Myeong-Seon Jeong,Joo-Eun Lee,Hyun-Suk Jung,Hyun-Joo Ro,Sangmi Jun,Wonku Kang,Seung-Yong Seo,Young-Eun Cho,Byoung-Joon Song,Moon-Chang Baek

Journal of virology 89:11523-33 PubMed26355084

2015

Cooperation between the Hepatitis C Virus p7 and NS5B Proteins Enhances Virion Infectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Mounavya Aligeti,Allison Roder,Stacy M Horner

View all publications

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Sphingomyelin Assay Kit (Fluorometric)