Soluble Epoxide Hydrolase Assay Kit (ab240999)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Purified protein, Tissue Lysate
製品の概要
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製品名
Soluble Epoxide Hydrolase Assay Kit -
検出方法
Fluorescent -
サンプルの種類
Cell Lysate, Purified protein, Tissue Lysate -
製品の概要
Soluble Epoxide Hydrolase Assay Kit (ab240999) is a microplate based fluorometric kit for measuring Soluble Epoxide Hydrolase (sEH) activity in cells and tissues as well as purified protein. It is based on the ability of sEH to hydrolyze a non-fluorescent substrate to a fluorescent product. The kit includes a specific inhibitor for soluble epoxide hydrolase, since the substrate can be hydrolyzed by non-specific hydrolases that are present in cell and tissue lysates. Specific sEH activity can be obtained by subtracting the activity in presence of sEH inhibitor form the total activity.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K477 Soluble Epoxide Hydrolase Activity Assay Kit (Fluorometric). K477-100 is the same size as the 100 test size of ab240999.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Fluorescence Standard III 1 vial sEH Assay Buffer 1 x 25ml sEH Inhibitor 1 x 100µl sEH Positive Control 1 vial sEH Substrate 1 x 200µl -
研究分野
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機能
Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. -
組織特異性
Found in liver. -
関連疾患
Note=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. -
配列類似性
Belongs to the peptidase S33 family. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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別名
- EPHX
- EPHX 1
- Ephx1
see all
画像
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Fluorescence standard curve for sEH metabolite
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sEH activity in rat liver tissue lysate in absence and presence of inhibitor. -
Enzyme kinetics in presence and absence of inhibitor for sEH positive control, and rat liver lysate (3.6 µg protein per well).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab240999 は論文での使用が確認できていません。