Pyruvate Kinase Assay Kit (ab83432)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Adherent cells, Cell culture supernatant, Other biological fluids, Plasma, Serum, Suspension cells, Tissue
- Sensitivity: 0.1 mU/ml
製品の概要
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製品名
Pyruvate Kinase Assay Kit -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Enzyme activity (quantitative) -
検出感度
= 0.1 mU/ml -
全工程の試験時間
0h 40m -
製品の概要
Pyruvate Kinase Assay Kit ab83432 provides a simple, direct and automation-ready assay for measuring pyruvate kinase activity in cell culture supernatant, plasma, serum, tissue, urine and other biological fluids (not validated for use with whole blood).
How the assay works
In the pyruvate kinase assay protocol, PEP and ADP are catalyzed by pyruvate kinase to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (absorbance 570 nm) and fluorescence (Ex/Em 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured.
The kit detects 0.1 mU/ml pyruvate kinase.
Pyruvate kinase assay protocol summary
- Add samples and standards to wells
- Add reaction mix
- Analyze with microplate reader immediately and after 10-20 min
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K709 Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit. K709-100 is the same size as the 100 test size of ab83432. -
特記事項
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2000 tests Development Enzyme Mix I 1 vial 20 vials OxiRed Probe 1 x 200µl 20 x 200µl PK Assay Buffer 1 x 25ml 20 x 25ml PK Positive Control 1 vial 20 vials PK Substrate Mix 1 vial 20 vials Pyruvate Standard 1 x 100µl 20 x 100µl -
研究分野
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関連性
Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. -
細胞内局在
Cytoplasmic and Nuclear -
別名
- CTHBP
- EC 2.7.1.40
- MGC3932
see all
画像
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Functional Studies - Pyruvate Kinase (PK) Assay Kit (ab83432)Image from Liu J et al., PLoS One. 2014;9(3):e91606. Fig S2(A).; doi: 10.1371/journal.pone.0091606. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
PC-3 and MCF-7 cells were treated with OA at the indicated doses and PK activity was determined 12 hr after OA treatment using ab83432. The average values of three independent experiments were shown as Mean ± SD. *, P<0.05, **, P<0.01.
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Functional Studies - Pyruvate Kinase (PK) Assay Kit (ab83432)Pyruvate measured in biological fluid after 2 min, 10 min and 20 min incubation time showing quantity (nmol) per ml of tested sample.
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Functional Studies - Pyruvate Kinase (PK) Assay Kit (ab83432)Pyruvate measured in mouse tissue lysates after 2 min, 10 min and 20 min incubation time showing quantity (nmol) per mg of tested sample. -
Functional Studies - Pyruvate Kinase (PK) Assay Kit (ab83432)Pyruvate measured in cell lysates after 2 min, 10 min and 20 min incubation time showing quantity (nmol) per 1 mln of tested cells. -
Functional Studies - Pyruvate Kinase (PK) Assay Kit (ab83432)Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted. -
Functional Studies - Pyruvate Kinase (PK) Assay Kit (ab83432)Fluorometric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
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Functional Studies - Pyruvate Kinase (PK) Assay Kit (ab83432)Pyruvate kinase samples time line using ab83432. -
Functional Studies - Pyruvate Kinase (PK) Assay Kit (ab83432)Pyruvate standard curve using ab83432.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (37)
ab83432 は 37 報の論文で使用されています。
- Hernandez-Espinosa DR et al. Microglial reprogramming by Hv1 antagonism protects neurons from inflammatory and glutamate toxicity. J Neurochem 165:29-54 (2023). PubMed: 36625847
- Rajabian N et al. Methionine adenosyltransferase2A inhibition restores metabolism to improve regenerative capacity and strength of aged skeletal muscle. Nat Commun 14:886 (2023). PubMed: 36797255
- Kogot-Levin A et al. Mapping the metabolic reprogramming induced by sodium-glucose cotransporter 2 inhibition. JCI Insight 8:N/A (2023). PubMed: 36809274
- Kuo CW et al. Pyruvate kinase M2 modification by a lipid peroxidation byproduct acrolein contributes to kidney fibrosis. Front Med (Lausanne) 10:1151359 (2023). PubMed: 37007793
- Minic Z et al. Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes. Biomedicines 11:N/A (2023). PubMed: 37189694