Global Protein Synthesis Assay Kit

• Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Protein Synthesis Assay Kit (Green, Microplate) (AB273286)

Metabolic labeling of protein on proliferating HeLa cells. 1 x 10⁵ cells incubated overnight with fresh aliquots of media containing Protein Label : Upper panel corresponds green fluorescence of de novo synthesized peptides. The lower panel shows panel cells treated with 1X Cycloheximide. Nuclear staining in both panels confirms that green signal is a result of Protein Label incorporation.

• FuncS

Supplier Data

Functional Studies - Protein Synthesis Assay Kit (Green, Microplate) (AB273286)

Plate reader analyses on Jurkat cells.

Jurkat cells : plate reader analyses of controls and Cycloheximide treatment; Avg fluorescence +/- standard deviation plotted for 3 replicates per condition.

• FuncS

Supplier Data

Functional Studies - Protein Synthesis Assay Kit (Green, Microplate) (AB273286)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Fluorescence azide curve of Jurkat cells prepared for this assay. Detection limit corresponds to about 31,250 of Jurkat cells per well.

• FuncS

Supplier Data

Functional Studies - Protein Synthesis Assay Kit (Green, Microplate) (AB273286)

Azide Fluorescence Curve in 0-0.1 X range.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

This is reference data and it should not be used to interpret actual results. Your data will depend on the cell type and tested compound.

• Schematic Diagram

Supplier Data

Schematic Diagram - Protein Synthesis Assay Kit (Green, Microplate) (AB273286)

Representative image of Global Protein Synthesis Assay Kit ab273286

Components shown from left to right :

- 400X Protein Label

- 1000X DAPI

- 100X Cycloheximide

- Fluorescent Azide II

- Reducing Agent

- 100X Copper Reagent

- Fixative Solution I

- 10X Permeabilization Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.